Effect Of Xuanfei Pingchuan Recipe On IL-6, ET-1 And TNF-α In Serum Of Asthmatic Rats

Purpose：In the establishment of rat model of bronchial asthma，based on Xuan FeiPing Chuan Decoction given intervention.Observation the changes of pathologicalmorphology of rat lung tissue of bronchial asthma research，and blood of therats of interleukin-6、Endothelin1and Tumor necrosis factorα. And a controlgroup for comparison. perspectives of Xuan Fei Ping Chuan Decoction of Chinesemedicine treatment of bronchial asthma rats model of the mechanism，for thetreatment of asthma medication and provide experimental evidence．Material and method：1Adaptive fed the rats for2days to adapt to the laboratorye environment. Inthis experiment according to the weight check random number table were randomLydivided into four groups，the blank group，model group,the Traditional Chinesemedicine group and the western medicine group．2The preparation of asthma model in rats：In addition to the blank group, herest three groups,in experiment1and8days to intraperitoneal injection inrats10%ovalbumin, aluminum hydroxide mixture1mL. On both sides of the waistto each1point at the same time, each point of subcutaneous injection of0.5mL10%ovalbumin and mixture of aluminum hydroxide, a total of2mL, injectionsensitization of rats.15th day in the experiment to asthma spray excitationin rats, with40mL3%of ovalbumin spray, spray flow control in5mL/min, everytime15minutes or cause symptoms such as shortness of breath, difficulty, onceper day, a total of3weeks. Blank group with normal saline instead of ovalbuminon intraperitoneal injection and atomization inhalation.3Lavage intervention：the blank group、model group giving each physiologicalsaline1mL/100g/times，2times/d, the Traditional Chinese medicine group giving Xuan Fei Ping Chuan Decoction,0.9mL/100g/times，2times/d, the western medicinegroup giving dexamethasone,0.5mL/100g/times，2times/d.4The experimental indicators drawn and detection4.1Observation of the general statust.During the experiment, diet, mentalstatus after processing,the change in color,weight deaths and so on wereobserved in rats。4.2After the experiment,each experimental group rats were fed nothing butwater for12hours. anesthesia,cut the rat’s chest cavity. About separation,exposed the main bronchus, quickly ligation, right main bronchus in the middleof the right lung tissue.Abdominal aorta take blood interleukin-6under test,and endothelin1and tumor necrosis factorα.Results：1The success of rat model of bronchial asthma.2Xuan Fei Ping Chuan Decoction bronchial asthma can significantly improvethe overall state of rats to reduce the bronchial and lung tissue inflammationand airway infiltration cramps，from organizational structure to reduce theextent of bronch ial asthma.3Xuan Fei Ping Chuan Decoction may reduce the IL-6、ET-1and TNF-αexpression，improve bronchial and lung tissue inflammation and airway spasm infiltration，reducing the degree of bronchial asthma，and inflammatory depth factor aspectsof the pathogenesis of bronchial asthma。Conclusion：1Multi-point subcutaneous injection using ovalbumin(OVA)approach，the successof induced rat model of bronchial asthma．2The traditional Chinese medicine group significantly improved the generalstate of rats to reduce the lung tissue of the degree of inflammatory ceilinfiItration，which Chinese traditional medicine group and the western medicinegroup no significant difference． 3Xuan Fei Ping Chuan Decoction rat soup may lower blood IL-6，ET-1and TNF-αlevels，with the effect of dexamethasone line close．Through the treatmentprocess can reduce the airway and lung tissue infiltration of inflammatorycytokines，improve alveolitis，effective to alleviate airway edema，convulsionsnarrow．Inflammatory factors from the perspective of depth Xuan Fei Ping ChuanDecoction treatment of bronchial asthma pathogenesis．...