Effects Of Iodine - Supplemented Chinese Herbal Compound And Iodine Excess On The Differentiation Of Thyroid Th17 Cells In Iodine Deficient NOD.H-2 ^H4 Mice

Purpose:To compare the effect of iodine-rich herbal decoction and iodine excess on theinflammatory axis of IL-23/IL-17cells in NOD.H-2h4mice with deficiency iodine.Material and method:8-week-old NOD.H-2h4female mice were made common animal model of susceptibleautoimmune thyroiditis and goiter caused by iodine deficiency by feeding a lowiodine diet and double distilled water with90days,randomly divided into fivegroups after the success of modeling: the normal control group(NC)﹑the modelcontrol group(MC)﹑the iodine-rich herbal decoction group[(HIE)Theingredients of HIE group include Haizao15g, Kunbu15g, Haidai7.5g with theiodine concentration of1900.36μg/L, take decocted, Concentrated medicinalbroth to make crude drug concentration was100%, According to the same surfacearea with people convert irrigation dose, Crude drug content of4.7g/(Kg·d)]and the iodine excess group[(IE) Contains iodine1900ug/L of deionized water,Prepared with potassium iodate]. After90days of giving treatment factors,themice were killed.The levels of serum total triiodothyronine(TT3)andtotalthyroxine(TT4) were measured by radioimmunoassay(RIA).Meanwhile themice serum thyroid stimulating hormone(TSH)were measured byradioimmunoassay(IRMA).The morphology of thyroid cells were observed underoptical microscopy.The expression of CD68in thyroid stroma cells and IL-17weredetected with immunohistochemical method.The expression of IL-17mRNA and IL-23mRNA in the thyroid of NOD.H-2h4mice were determined by RT-PCR method.Theexpression of IL-17and IL-23in the thyroid of NOD.H-2h4mice were determinedby the Western blot method. Result:1.90days after treatment by different factors, Thyroid function in mice: thenormal control group(NC) serum TSH（1.14±0.23mUI/L）, serum TT3（2.24±0.54nmol/L），serum TT4（90.86±9.35nmol/L）.the model control group(MC) serumTSH（1.33±0.13mUI/L），serum TT3（2.36±0.72nmol/L），serum TT4（167.68±68.74nmol/L）.the iodine excess group(IE) serum TSH（1.33±0.07mUI/L），serumTT3（2.44±0.85nmol/L），serum TT4（117.79±21.98nmol/L）.the iodine-rich herbaldecoction group(HIE) serum TSH（1.24±0.25mUI/L）, serum TT3（3.35±0.54nmol/L），serum TT4（130.46±42.85nmol/L）. There was not obvious difference among everygroup on serum TT3,TT4and TSH (P﹥0.05).2.The light microscope Result: the normal control group(NC)；Cubic shaped thyroidepithelial cells, follicular cavity is relatively homogeneous medium size,uniform lumen glial rich, give treatment factor90days have two mice spontaneousthyroiditis (2/l0), and minor inflammation, others are no thyroiditis. Modelcontrol group (NC)；Thyroid follicular thyroid tissue morphology change is notobvious, size is differ, in the central part of the right size, is relativelyuniform, surrounding some larger follicle, follicular epithelial cells,columnar, nuclear round or oval, follicular cavity with the same amount of pinkcolloid，For processing factors of90days, only have1mice of colloid goiteroutside, the rest of the9are not happen thyroiditis (0/10). iodine excess group(IE); Follicular cavity increases, colloid filling, extruding flat epithelialcells, inflammatory cells infiltration, thyroid and infiltration area of totalarea of about50﹪, give treatment factor90days except1mice did not happenedthyroiditis,others all happened thyroiditis(9/10). The iodine-rich herbaldecoction group(HIE);With follicular thyroid follicular epithelial cells showcolumnar or cuboidal and colloid in follicular cavity filling give treatmentfactor90days, Although with inflammatory cells infiltration, infiltration areais lesser, And only3mice occur thyroiditis (3/10). The results showed theiodine-rich herbal decoction group compare with iodine excess the incidence ofautoimmune thyroiditis NOD mice can be significantly reduced，Proved that theiodine-rich herbal decoction group did not like the iodine excess increasing autoimmune thyroiditis lymphocyte infiltration.3.Immunohistochemical determination results：90days after treatment bydifferent factors, Compared with the iodine excess group (IE), the expressionof CD68in thyroid stroma cells in the iodine-rich herbal decoction group(HIE)was significantly decreased (the integrated optical density:0.13±0.06vs0.28±0.07)(P﹤0.01) and the expression of IL-17in thyroid tissue in iodine-richherbal decoction group(HIE) was obviously decreased(the integrated opticaldensity:0.19±0.05vs0.21±0.07)(P﹤0.01). This shows compared with the iodineexcess group (IE), the expression of CD68in thyroid stroma cells and theexpression of IL-17in thyroid tissue in the iodine-rich herbal decoctiongroup(HIE) was significantly decreased(P﹤0.01).4.RT-PCR: The mean relative expression of IL-17mRNA and IL-23mRNA in theiodine-rich herbal decoction group(HIE)(1.31±0.06，1.43±0.50),issignificantly less than in the iodine excess group (IE)（2.39±0.05，2.21±0.70）(P﹤0.05）.5.Westernblot: The protein expression of IL-17and IL-23in the iodine-richherbal decoction group(HIE)（0.73±0.02，0.27±0.02）, is significantly lessthan in the IE group(0.80±0.03,0.36±0.03)(P﹤0.05).Conclusions:Compared with the iodine excess, rich iodine Chinese herbal medicine decoctionmay obviously reduce the incidence of autoimmune thyroiditis in iodinedeficiency NOD.H-2h4mice，and the mechanism may be related to obvious inhibitionof high expression of IL–23and IL–17in thyroid.