Experimental Study On Effects Of Yiqi Huoxue Recipe On Myocardial Energy Metabolism In Chronic Heart Failure Rats

Purpose: This research through the rat model of chronic heart failure after filling Yiqi huoxue Compound and lisinopril tablet, explore the effect on chronic heart failure rat of Yiqi huoxue Compound of myocardial energy metabolism, make the PGC-1α、NRF-1、mt TFA、ATP as the basis, discuss the related mechanism and targets, for the motherland medicine will provide a scientific basis for prevention and treatment of chronic heart failure failure and experimental method.Material and method: 1 To established the Chronic heart failure rat model:Choose SPF 12 weeks healthy SD rats, male, 80, in 250±10 g weight,by ligation of left anterior descending coronary artery,cooperate with swimming exhaustion type and the method of dieting to established the Chronic heart failure rat model. According to dynamic electrocardiogram(ecg) during coronary artery ligation of chest lead ST segment elevation, after building the computed echocardiographic left ventricular ejection fraction(EF values≤50%), as a symbol of building successful chronic heart failure.The model rats with depression, claw back petechial edema of hair removal Throat rale and other signs for distinguishing between qi deficiency and blood stasis card of water stop. 2 Animal experiment grouping: 43 will only build mould successful rats using random Numbers table find residual method is divided into: model group(14), Chinese medicine group(15), western medicine group(14). At this point, the normal group(control group) randomly to return 15 only to try to control the balance between groups of sample size. 3 Xperimental drugs: yiqi huoxue compound composition: radix astragali, radix pseudostellariae, safflower, red sage root, motherwort, poria cocos, semen lepidii. By Liaoning University of traditional Chinese’s hospital medicine pharmaceutical center and preparation. Western drugs lisinopril pieces produced by Shanghai sym vientiane pharmaceutical co., LTD., the specification for each piece of 10 mg, the batch number is: HI0960265. 4 Experimental drug doses : normal group and model group rats, daily to give volume distilled water to fill the stomach, such as Chinese traditional medicine group according to the student the dose of 9.2 g/(kg. d) give Yiqi huoxue compound lavage, western medicine group according to the student the dose of 1.5 mg/(kg. d) given lisinopril lavage, continuous dosing 28 d. 5 Eal-time fluorescent quantitative PCR technology in each rat myocardial tissue, mt TFA、PGC-1 alpha, the NRF- 1 m RNA expression level. 6 Protein immunoblot technology(Western blot) in the observation group rats myocardial tissue, mt TFA、the NRF- 1 the change of protein expression, and average gray level values are measured. 7 Colorimetric method for determining the concentration of ATP levels, ELISA method for determining the concentration of BNP levels. 8 the microscopic observation of myocardial mitochondrial changes in the cell.Results: 1 Compared with normal group(control group), the treatment group(traditional Chinese medicine, western medicine group) and BNP concentration in model group were increased, p = 0.000(p < 0.01) and significant difference between groups, prompt myocardial tissue in heart failure within the BNP levels showed a trend of rise. In the treatment group compared with model group, treatment group BNP concentration decreased, p = 0.000(p < 0.01) and significant difference between groups, explains the experimental drug therapy to reduce the BNP. The BNP concentration in the traditional Chinese medicine and western medicine group comparison, p = 0.691(p > 0.05), there was no statistically significant difference between the groups, notes, the curative effect of western medicine. 2 Compared with normal group, the ATP concentration in the treatment group and model group were reduced, p = 0.000(p < 0.01) and significant difference between groups, hints of ATP content in myocardial tissue in heart failure showed a trend of decrease. Compared with model group, the ATP concentration in treatment group were higher, p = 0.000(p < 0.01) and significant difference between groups, explains the experimental drug treatment can increase the ATP content in myocardial tissue, promote the release of energy; Traditional Chinese medicine and western medicine group ATP concentration, p = 0.159(p > 0.05), there was no statistically significant difference between the groups, notes, the curative effect of western medicine. 3 Compared with normal group, the treatment group and model group, NTF- 1 、 mt TFA m RNA expression and protein expression are lower, p = 0.000(p < 0.01) and significant difference between groups, prompt myocardial tissues in heart failure, NTF- 1、mt TFA content showed a trend of decrease. Compared with model group, treatment group of NTF- 1 、mt TFA m RNA expression and protein expression are higher,(p = 0.000 < 0.01) and significant difference between groups, suggests that the treatment group of drugs can improve the myocardial tissue of NTF- 1, the content of mt TFA prompts the experimental drug treatment can increase the generation of mitochondrial。 4 Observation under electron microscopy(sem) in the myocardial mitochondria that: normal group in the rat myocardial mitochondria in cells abundance, shape size, ridge structures such as can be clearly seen in the mitochondria, mitochondrial outer membrane smooth and no damage, no swelling of mitochondria; Model group rats myocardial mitochondria in cells is relatively fewer in number form size is irregular, mitochondria swelling, ridge in the mitochondria structure such as texture disorder is not clear, even form only frame structure of vacuoles, mitochondria mitochondrial outer membrane damage, even fracture; Western medicine group in the rat myocardial mitochondria in cells compared with model group increased number, but it is still less than normal group, although mitochondrial abnormalities, such as size of irregular form, but the overall shape size, mitochondrial cavitation abnormal mitochondria structure to reduce in the model group; Chinese traditional medicine group in the rat myocardial mitochondria in cells number less than western medicine group, but more than the model group, the mitochondrial cavitation, the swelling deformation, spinal disorders, such as abnormal structure compared with model group decreased. Instructions, yiqi huoxue compound can optimize the proliferation of mitochondria, improve the abnormal mitochondria structure, group and western medicine and Chinese medicine treatment group.Conclusion: 1 By comparing the data, found that the content of ATP and the NRF- 1, mt TFA reduced expression in the process of chronic heart failure, heart failure happened when there exists a mitochondrial energy metabolism disorder. 2 Through the comparison between each group, the microscopic microstructure change of mitochondria, prove that in the presence of abnormal mitochondria damage in heart failure, compared with model group, the experimental drug on mitochondria have good curative effect. 3 By comparing the results between groups that yiqi huoxue compound is able to improve myocardial energy metabolism, the formulas by increasing NRF- 1、 the expression of mt TFA,increase grain with generation and ATP production capacity, improve myocardial energy metabolism, inhibit the action of the ventricular remodeling.