Study On The Changes Of Serum FKN In Rats At Different Stages Of Acute Pancreatitis By Salvia Miltiorrhiza Injection

Purpose:In this experiment, clean Wistar male healthy rats as the experimental object of study, induced acute pancreatitis model method, take along the tail intravenous Danshen injection, observation and comparison in different periods of acute pancreatitis, application of Salvia miltiorrhiza injection on rat serum chemotactic factor(fractalkine FKN) effect and the change of the serum amylase, with its through the experimental study to further clarify the mechanism of transformation of Danshen injection in prevention of acute pancreatitis and prevention of edema type pancreatitis to necrotizing pancreatitis.Materials and methods:The rats aged 4-5 weeks of Wistar series clean grade healthy male rats were randomly divided into 6 groups according to the number, 10 rats in each group, the body weight of 280 g + 20 g requirements. According to Mizunuma T[1]、Yang Ping[2]、Yu Zhonghang [3] literature describes methods used, small dose(2mg/g) of 10%L- arginine solution for intraperitoneal injection method to copy the model of acute pancreatitis in rats, according to the different time(before modeling for 30 minutes, modeling, model after 30 minutes, after modeling for 60 minutes, take) intravenous injection of Danshen injection(36mg/kg * weight) solution until the end. Rats were anesthetized by intraperitoneal injection of anesthesia to take, 5ml dry clean sterile syringe aortic 3m L/ only. Observation of serum amylase(colorimetric method), chemokine(FKN, ELISA). The experimental rats were fasted for 12 hours before drinking, no limit. The experimental group rats were used for the L- method, the drug injection amino acid solution, the application of 2.0mg/g dose index for the medicine. 0.9% sodium chloride injection amount given to the control group, the treatment group according to the time of administration is divided into four groups, treated respectivelyas: treatment of the 1 groups, in the rat model of acute pancreatitis were prepared from 30 minutes ago, tail intravenous injection of Danshen injection, to the end of the experiment, the dosage standard for 36mg/Kg. Treatment group 2, model preparation and tail intravenous injection of Danshen injection, to the end of the experiment, the dosage standard for 36mg/Kg. Treatment group 3, model preparation after 30 minutes, the tail intravenous injection of Danshen injection, to the end of the experiment, the dosage standard for 36mg/Kg. Treatment group 4, model preparation after 1 hours, the tail intravenous injection of Danshen injection, to the end of the experiment, the dosage standard for 36mg/Kg. Modeling for 2 hours after the abdominal aortic blood, anesthesia by intraperitoneal injection of 20% urethane, 5ml dry clean sterile syringe aortic blood samples of 3m L/ only, centrifuge for 20 minutes, the requirements of 3000 rpm, separating serum.-20 degrees Celsius to save, the content of serum amylase and FKN were measured respectively.Results:1.Effect of serum amylase In Group level compared with the blank control group, the level of serum amylase(P<0.01), the model significantly increased amylase levels in serum of Radix Salviae Miltiorrhizae group increased significantly(P<0.01); compared with the model group, Salvia miltiorrhiza treatment group 1, treatment group 2, Salvia miltiorrhiza treatment amylase levels in the 3 groups were significantly decreased compared with the model group(P<0.O1), Salvia miltiorrhiza group 4 the amylase level than the level of amylase in model group had no significant decrease(P > 0.05), comparison of Salvia miltiorrhiza group, Salvia miltiorrhiza treatment were compared between the 1 groups of serum amylase level of treatment in the 2 groups decreased significantly(P<0.01), Salvia miltiorrhiza treatment in 2 groups were compared the serum amylase levels in the 3 groups decreased significantly(P<0.01), Salvia miltiorrhiza treatment in 3 groups were compared serum amylase levels in the 4 groups decreased significantly(P<0.01), tip: Salvia miltiorrhiza injection can reduce the serum amylase in acute pancreatitis rats, the sooner the use effect is more obvious.2 Effect on serum FKN The model group and Danshen group compared with blank control group, the level of FKN increased significantly(P<0.01), Salvia miltiorrhiza treatment group 1, treatment group 3 of 2 Salvia miltiorrhiza treatment group compared with the model group, the FKN level decreased significantly(P<0.01), Salvia miltiorrhiza group 4 FKN level is the level of FKN in model group had no significant decrease(P < 0.O5) comparison of Salvia miltiorrhiza group, Salvia miltiorrhiza Salvia miltiorrhiza treatment, compared 1 groups of serum FKN levels in 2 treatment group decreased significantly(P<0.01), Salvia miltiorrhiza treatment in 2 groups were compared the serum FKN levels in the 3 groups decreased significantly(P<0.01), Salvia miltiorrhiza treatment in 3 groups were compared the serum FKN levels in the 4 groups decreased significantly(P<0.01), tip: Salvia miltiorrhiza injection can decrease the level of serum FKN in rats with acute pancreatitis, the sooner the use effect is more obvious.Conclusion:1 Danshen injection can reduce the serum FKN content in rats. 2 Danshen injection can reduce the serum amylase content in rats. 3 Salvia miltiorrhiza injection can reduce the acute pancreatitis in rats serum amylase, FKN, use early in the decreasing amplitude is large.