Preparation Of Nano - Carboxymethyl Chitosan Sodium Fluoride Coating And Laboratory Study On Prevention Of Caries

Objective : To prepare nano carboxymethyl chitosan sodium fluoride varnish(NCCF varnish);to research the fluoride ion release of the varnish in vitro,the anti-caries characteristics of inhibition enamel demineralization and antimicrobial ability.Methods:Carboxymethyl chitosan was formulated into nanoparticles by the well-known ion-crosslinked method. The fluoride varnish was prepared from the developed sodium fluoride loaded carboxymethyl chitosan nanoparticle(CCS-SF-NPs) and self-etching adhesive;the release of fluoride varnish in vitro was evaluated by characterization of the cumulative release rate of fluoride ion;using the PH-cyling to creat the model of the enamel demineralization in vitro,and find the inhibition of NCCF varnish for enamel demineralization;the number of colonies was counted on HA dics to evaluate the inhibition of the growth and adhesion ability of the NCCF varnish by selecting three important cariogenic bacteria to culture in vitro, imitating oral environment.Results:The CCS-SF-NPs were presented spherical or spheroidal and the particle size averaged in(258.72±5.86)nm.Its encapsulation efficiency was 71.74% and drug loading were 11.01%. With compared the release time,the NCCF varnish showed significantly longer than the copal varnish.The dissolubility of the calcium from enamel of NCCF varnish group was significantly lower than that of the other fluoride materials(P<0.05);with compared the colony count of Streptococcus mutans and Streptococcus sanguis on HA discs, NCCF varnish showed significantly less than the control(P<0.05).Conclusion:The NCCF varnish shows not only excellent properties of fluoride release,but can it inhibit of enamel demineralization and antimicrobial ability such as the Streptococcus mutans and Streptococcus sanguis.It has the potential caries prevention in clinic application.