| Herba Artemisia capillaries is widely used in clinic,which is also be recorded in ChP as one kind of common Chinese traditional herb.The existing quality standards of Herba Artemisia capillarie are not perfected enough.The traditional description identification,microscopic identification and the Physical and Chemical identification are used to determine the quality of herbas.The first two methods belong to qualitative analysis,which are allways impacted by workers.The latest method only takes individual active components into account.They can not meet the requirements of quality control of traditional Chinese medicine modernization.The present study provides a method to determine the index components in Herba Artemisia capillaries by high performance liquid chromatography.Besides,HPLC fingerprint chromatogram and GC fingerprint chromatogram was established for the herb.（1）Chlorogenic acid,caffeic acid and 4-hydroxyacetophenone have been reporting as the effective components.We established a method for the determination of chlorogenic acid,coffeic acid and 4-hydroxyacetophenone in Herba Artemisia capillaries by RP-HPLC.The RP-HPLC method detected by different UV wavelengths—325 nm for chlorogenic acid and caffeic acid, 275 nm for 4-hydroxyacetophenone respectively had been developed:The system consisting of a C18 column（250mm×4.6mm,5μm）and a mixture of acetonitrile and 0.04%phosphate acid （9:91）as the mobile phase was adopted.The linear response ranges were 52.00～1.300x103μg.mL-1（r=0.9999）,1.600～40.00μg.mL-1（r=0.9999）and 0.6720～16.80μg·mL-1（r= 0.9995）,respectively（n=5）.The average recoveries（n=9）of chlorogenic acid,caffeic acid and 4-hydroxyacetophenone were 98.1%,98.6%and 100.8%,respectively.The assay demonstrated that the method had adequate accuracy and selectivity to measure the three chemical constituents in Herba Artemisia capillaries.（2）To establish a method for the determination of Rutin,Hyperin,Isoquercitrin,4, 5-O-dicaffeoyl quinic acid and Isorhamnetin-3-O-glucoside by RP-HPLC.The Rutin,Hyperin, Isoquercitrin,4,5-O-dicaffeoyl quinic acid and Isorhamnetin-3-o-rutinoside in the 11 batches of Herba Artemisia capillaries,were analyzed.The chromatographic condition included a Luna C18 column（250mm×4.6mm,5μm）and the mobile phase consisting of acetonitrile-0.04% phosphate acid（17:83）and the absorbance was monitored at 345 nm.The linear response ranges were 0.960～19.20μg.mL-1（r=0.9995）,1.200～24.00μg.mL-1（r=0.9995）,1.200～22.40μg.mL-1（r=0.9994）,56.00～1.120×103μg·mL-1（r=0.9990）and 0.6400～12.80μg·mL-1（r= 0.9998）,respectively（n=5）.The average recoveries（n=9）of rutin,hyperin,isoquercitrin,4, 5-O-dicaffeoyl quinic acid and isorhamnetin-3-O-glucoside were 98.1%,100.7%,98.4%, 100.2%and 101.7%,respectively.The assay demonstrated that the method had adequate accuracy and selectivity to measure the five chemical constituents in Herba Artemisia capillaries.（3）HPLC fingerprint chromatogram was established for Herba Artemisia capillaries.The condition included Luna C18（250mm×4.6mm i.d,5μm）column,the gradient elution with acetonitrile and 0.04%phosphate acid solution.The concentration of acetonitrile increases from 15%to 12%in 12 minutes,increases to 16%during,the later 5 minutes,increases to 25%in next 23 minutes,increases to 90%finally at the 90 minutes.The detection wavelength was 325 nm.15 batches of Herba Artemisia capillaries.were studied under this condition.The Ward’s method and the Euclidean distance were used as the measurement index to the fingerprints of Herba Artemisia capillaries.The Herba Artemisia capillaries,mutual pattern was established on the basis of the data from 11 batches of Herba Artemisia capillaries.Then the similarity was calculated respectively.This method can be validly used to evaluate the quality of Herba Artemisia capillaries.（4）GC fingerprint chromatogram was established for Herba Artemisia capillaries.The condition included PEG-200 capillary column（30m×0.25mm×0.25μm）,and the program was 50℃5℃/min 160℃3℃/min 235℃（15 min）with the FID detection.15 batches of Herba Artemisia capillaries.were studied under this condition.The Ward’s method and the Euclidean distance were used as the measurement index to the fingerprints of Herba Artemisia capillaries.The Herba Artemisia capillaries,mutual pattern was established on the basis of the data from 8 batches of Herba Artemisia capillaries.Then the similarity was calculated respectively.This method can be validly used to evaluate the quality of Herba Artemisia capillaries.
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