Study On Quality Standards And Fingerprint Chromatogram Of Radix Polygoni Multiflori And Radix Polygoni Mulitiflori Praeparata Cum Succo Glycines Sotae

Medical material of polygonum multiflorum thumb. is recorded by《hinese Pharmacopoeia》PartⅠ(2005 Edition), come from dry earthnut of Polygonum multiflorum. Chinese traditional medicine deems that polygonum multiflorum thumb.. have the quality of flat, bitter and a litter sweet,It have the effects of detoxification, eliminating carbuncle, defaecation. Hence, it is usually used as clinical turgescence and laxative. When polygonum multiflorum thumb. been processed, it becomes warmer and sweeter and has the effect of adding liver and kidney, profiting essence and blood, darkening beard and hair, strengthening bones and muscles. It is used clinically for therapy of tumefaction, carbuncle, rubella, itch, constipation, high blood lipids and other diseases. Main components of polygonum multiflorum thumb. are anthraquinones, toluylene glycosides, Phospholipids. Pharmacological experiments show that the anthraquinone in the Polygonum multiflorum have the effect of lowering cholesterol, reducing blood sugar, anti-virus, strengthening heart, promoting gastric peristalsis, etc, as well as promoting fibrinolytic activity and the preventive of cerebrovascular diseases; the lecithin ingredients are contained in brain tissue, blood cells and other cell membrane composition of the material, regular consumption of fleece-flower root, can cure neurasthenia, white hair, hair loss, anemia and other diseases, therefore, it can delay aging, promote physical fitness, protect the health of heart. Animal experiments show that there are anti-tumor effects of Polygonum multiflorum. There are strong role of fleece-flower root to strengthen nerves, so it can develop intelligence. It also can promote the growth and development of blood cells, there is a significant anti-aging effects.Traditional Chinese medicine in our country under the guidance of the theory of traditional Chinese medicine is used for diagnosis, treatment, prevention of illness, Chinese medicine think that it has the effect of therapy for the combined effects of various components in herb.Evaluation of drugs (including Chinese Herbal Pieces) Quality should be measured by three areas, namely, authenticity, purity and the quality of degrees. Authenticity should be evaluated through drug sources, properties and identification of projects; purity should be evaluated through the examination of moisture, ash, general contamination, special inspection and the measure of physical constants; high quality degrees should be evaluated through the measurement of extraction and content. Therefore, quality evaluation of Chinese medicine should be consistent with the integrity of the principles of Chinese medicine. Current national and ministerial pharmacopoeia standards on the assessment of the quality of fleece-flower root Pieces which adopt a single indicator is difficult to comprehensively and accurately evaluate the overall quality of herbs. This study aimed to combine a single component of the evaluation with overall evaluation of fingerprint, establish a comprehensive and rational system of quality assessment, provides a scientific basis for the standardization of medicinal plant, quality appraisal, the use of clinical medicine.The issue first gather and organize the information of polygonum multiflorum thunb to determine the pharmacological effects of its active ingredients and chemical properties, used to guide the polygonum multiflorum thumb. quality standards research. This study examined the medicine processing methods of polygonum multiflorum thunb, their traits, TLC method, the quality-related inspection items, HPLC methods, polygonum multiflorum thunb HPLC fingerprint of research method.Firstly, establish the character's microscope identification methods of Polygonum multiflorum thumb.Scondly, establish the relevant quality inspection items included the inspection of Moisture, ash, acid insoluble ash and extraction. Through the determination of different commercially available batches of 10 Pieces, the relevant limit standards are developed. Result:moisture is no more than 12%; ash is no more than 9%;acid insoluble ash is no more than 2.0%;the extraction of the raw is no less than 18%,and the prepared is no less than 5.0%.Thirdly, through the study of chromatographic conditions, establish a target for the evaluation of stilbene glucoside by HPLC assay methods:HPLC was used on a chromatographic column of A ichrom Bond-AQ C18(5μm,4.6mm×250mm);the detection wavelength was at 320nm; acetonitrile-water(25:75) solution was taken as gradient elution;the flow rate was 1.0 mL/min;the column temperature was 30℃,and the analysis time was 10min. Through the determination of different commercially available batches of 10 Pieces, the relevant content limits standards are developed:the stilbene glucoside of raw Polygonum multiflorum thumb. is no less than 1.0%, and the prepared is no less than 0.7%.Forthly, through the research of 10 different groups of polygonum multiflorum thumb. come from various habits by the method of HPLC-fingerprint, establish the HPLC-fingerprint of raw polygonum multiflorum thumb., determine 13 mutual peaks on the chrom autographic fingerprint, the similarities were more than 0.9; establish the HPLC-fingerprint of prepared polygonum multiflorum thumb., determine 10 mutual peaks on the chrom autographic fingerprint, the similarities were more than 0.8;Through the topics, the quality standard of polygonum multiflorum thumb. is established.