| Mycotoxins can cause serious health hazards. Over 400 mycotoxins are known of which aflatoxins, trichothecenes, fumonisins, ochratoxin A and zearalenone are the main representatives. Aflatoxins are toxins with great accumulative toxicity and pose a significant potential threat to human and animal liver, which will lead to liver cancer or even to death. Ochratoxin A is a potent nephrotoxin and hepatotoxin with teratogenic, utagenic, carcinogenic and immunosuppressive effects even at race levels. Fusariums occur in different environment, which not only causes human and livestock acute and chronic poisoning, but also exist potential carcinogenic, aberrance and mutagenic. So people's health are influenced seriously by the residue of mycotoxins in food derived from animals. The quantification and confirmation methods should be established to provide the implements and means for solving their residue problem. Several analytical methods have been published for determination of mycotoxins in cereals, feeds, foods and biological fluids, but few HPLC-MS/MS method is so far established for the determination of Aflatoxins, Ochratoxin A and Fusariums in all kinds of animal derived food. Pre-treatment such as ultrasonic, homogenizing, and shaking are traditional techniques used for the extraction of mycotoxin in food and feed. However, they are time-consuming, cost a lot of organic solvents, and have a low efficiency in the extraction of residues. Accelerated solvent extraction (ASE) is a newly developed extraction technique presenting advantages of higher extraction efficiency, low cost of extraction solvent, and short time of analysis. It has already been used for the extraction of residues in soils, foods, drugs, and so on. Up to now, no applications have been reported using ASE for the simultaneous extraction of AFB1,AFB2,AFG1,AFG2, AFM1,AFM2, and OTA in animal derived food.So the purpose is to establish the multi-residue quantification and confirmation methods of Aflatoxins, Ochratoxin A and Fusariums to monitor the residue of these mycotoxins in animal edible tissues. The first method was developed for the determination of aflatoxins B1, B2, G1, G2, M1, M2 and Ochratoxin A residues in swine, cattle, sheep muscle, liver, kidney, fat, chicken muscle and liver, fish muscle and skin, eggs and milk, as well as feed. The target analytes were extracted with the solution of acetonitrile/ hexane (60/40, v/v) using accelerated solvent extraction (ASE) and then purified using solid-phase clean up (HLB) to eliminate most of the impurity.The quantitative analysis was performed with liquid chromatography-tandem mass spectrometric with electrospray ionization method. The limits of decision varied from 0.07 to 0.59μg/kg, the limits of detection varied from 0.20 to 1.21μg/kg (defined in terms of CCα). Within the spiked level of quantitative limits, the recoveries for most pesticides were between 69.4%-105.7%, the relative standard deviations were less than 17.6%.The second method is to develop a simultaneous method for the determination of fusariums including T-2 toxin, HT-2 toxin, T-2 triol, T-2 tetraol, DOM-1, Diacetoxyscirpenol, Monoacetoxyscirpenol, Neosolaniol, Deoxynivalenol, Nivalenol, 3-acetoxy deoxynivalenol,15-acetoxy deoxynivalenol, Fusarenon-x, Zearalanone, a-Zearalanol,β-Zearalanol, a-Zearalenol,β-Zearalenol, Zearalenone in swine, bovine and sheep muscle, liver, and kidney, chicken and fish muscle, eggs and milk, as well as feed. The samples were incubated with 100μlβ-glucuronidase solution. After enzymolysis, the samples extracted with acetonitrile and water, and hexane was used to remove the fat. Then the sample was evaporated at 45℃until the hexane was removed completely. The clean up was carried out in the Bond Elut Mycotoxin column. The residual was dissolved and then detected.CCαwere 0.10μg/kg-1.37μg/kg and CCβwere 0.37μg/kg-2.41μg/kg, respectively. The recoveries of fusariums, within the spiked level of quantitative limits, were from 64.8% to 105.0%. All the coefficient variations were≤18.6%.The conditions of sample preparation and detection by chromatography and mass-spectrometer were optimized in this study. This method is precise, sensitive and highly efficient in extraction. After routine applications, the results indicated that this method is suitable for the determination of aflatoxins, ochratoxin A and fusariums residues in various kinds of animal derived food. Meanwhile, the method successfully fulfilled the minimum limiting level requests from various countries. The development of methods provides technical support for monitoring residues of these mycotoxins in animal derived food.
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