Identification And Characterization Of Active Components From Magnolia

Magnolia belongs to Magnolia officinalis Rehd.et Wils. The dried stem bark, root bark and branch bark of magnolia, as a traditional Chinese medicine, not only have the pharmacological activities of anti-inflammatory, anti-aging and anti-oxidant, but also have a wide range activety of anti-bacterial. This paper focused on the effective antibacterial constituents of Magnolia officinalis, as well as the explore of the mechanism of its antibacterial.Antifungal activities of ethanol extracts from Magnolia were tested against Staphylococcus aureus(SA), Klebsiella pneumoniae, Escherichia coli, Alexandre Yersin, Pasteurella multocida, Listeria monocytogenes, Candida albicans, Aspergillus niger. The results show that the Magnolia has the strongest inhibition against SA. SA is not only the most common pyogenic bacteria, but also the main pathogens of pulmonary infection.Isolation and purification of the effective antimicrobial constituents from Magnolia officinalis by high performance liquid chromatography and high-speed countercurrent chromatogramphy was established. The two active components magnolol (5,5 - diallyl 2,2 '- dihydroxybiphenyl) and honokiol (3,5 - 2 -2 - propenyl 1,1' - United benzene, 2,4 '- diol), whose structures were identited by 1H nuclear magnetic resonance(NMR) and 13C NMR. The two components showed the same MIC of 6.25μg/ml. Through the experiments of Epistasis Mini-Array Profiling(E-MAP), magnolol and honokiol shows bacteriostatic activity against Staphylococcus aureus partly by inhibiting the synthesis enzymes of cell wall.A stably transfected Fischer thyroid epithelial (FRT) cell lines co-expressing wt-CFTR and a green fluorescent protein mutant with ultra-high halide sensitivity (EYFP-H148Q) was generated as assay for the drug screening. Using this cell model, magnolol and honokiol were screened for CFTR-mediated halide transport.Through these experiments, it suggested that the active ingredients in Magnolia can activate channels and inhibit inflammation. This finding provide a new chemical structures for the treatment of cystic fibrosis.