Establishment Of Solution Culture System For Moso Bamboo (phyllostachys Pubescens) Seedlings

Solution culture experiments were carried out on moso bamboo seedlings to establish a solution culture protocol. By compared of increased biomass, leaf-area, root/shoot, chlorophyll fluorescence characteristics and reflectance spectra characteristics, we selected optimal conditions, studied the effect of different N, P, K concentration in solution .The main findings are as follows:1. Experiment of selecting different formula of nutrient liquid treatments indected that the icreased biomass of CK similar to Yoshida treatment but significantly higher than Hoagland and MS treatments. The leaf-area, root/shoot, Chlorophyll fluorescence parameters and reflectance spectra characteristics of Yoshida treatment were superior to Hoagland and MS treatments. So, Yoshida formula of nutrient is more suitable for growth of moso bamboo seedlings.2. Experiment of selecting 1/4 Yoshida, 1/2 Yoshida, Yoshida, 3/2 Yoshida indected that with the increase of ionic strength the increased biomass, leaf-area and root/shoot performanced increased first and then decreased.1/2 Yoshida handling the peak. Chlorophyll fluorescence parameters and reflectance spectra characteristics of 1/2 Yoshida treatment were superior to other treatments, moreover Fm, Fv, Fv/Fm, Fv/Fo were very significantly. So, 1/2 Yoshida is better.3. Experiment of solution pH indected that the increased biomass of pH5.0 treatment significantly higher than pH4.0, pH4.5, pH5.5 and pH6.0 treatments.while leaf-area is very significantly higher. But root/shoot has no difference between treatments. Chlorophyll fluorescence parameters and reflectance spectra characteristics of 1/2 Yoshida treatment were superior to other treatments, meanwhile Fm, Fv, Fv/Fm, Fv/Fo were very significantly. So, the nutrient solution of pH5.0 is most suitable for growth of moso bamboo seedlings.4. Experiment of selecting interval of renew nutrition solution and optimal aeration time. By considering for the indexes of increased biomass, leaf-area, root/shoot, Chlorophyll fluorescence parameters and reflectance spectra characteristics, the renew nutrition solution per week was superior to 3d, 10d, 15d treatments.and aeration time 24h per day was superior to 8h and 16h treatments. So the interval of renew nutrition solution per week and aeration time 24h per day are most suitable for growth of moso bamboo seedlings.5. N, P, K univariate experiments. Experiment of selecting nitrogen concentration indicted that the indexes of 3mM treatment were superior to 1mM, 2mM, 4mM and 5mM treatments. Experiment of selecting phosphorus concentration indicted that the indexes of 1mM were superior to 0.5mM, 1.5mM and 2mM treatments. Experiment of selecting potassium concentration indicted that the indexes of 1.5mM superior to 0.5mM,1mM,2mM and 2.5mM treatments.6. N, P, K Orthogonal Test (L9(3~3)) shows that the optimum N, P, K levels were as follows:4mM nitrogen, 0.5mM phosphorus, and 1mM potassium were the best for the seedling growth.