S-genotypes Identification Of Prunus Simonii., Prunus Salicilia., Prunus Armeniaca. And The Application On Parent Selection Of Interspecific Hybridization

There are many new cultivers have been breeded through interspecific hybridization such as Prunus simonii.The new hybrid species shows excllence characteristics in fruit quality.But for international protection of intellectual property right,these cultivers can not been planted widely in China. As a result,breed new cultivars by using prunus salicilia and prunus armennion cultivars in domestic was a good choice to overcome this barrier. Self-incompatibility is another fatal problem in this way. One of the reason lead to Self-incompatibility is S-genotypes of the cultivars.Furthermore, Self-incompatibility also has influence on production of prunus salicilia and prunus armennion.So identify the cultivars' S-genotypes is the key to these questions.This study choose 38 prunus salicilia cultivars,18 prunus armennion cultivars and 7 prunus simonii cultivars as experimental material,63 cultivars' S-genotypes has been identified,15 new S-RNase gene from pistil and 3 new SFB gene from pollen have been isolation and identification.In addition,on the basis of cultivars' S-genotypes and mean fruit weight and fruit quality,use multivariate statistical analysis software do cluster analysis on 63 cultivars.Conprehensive flowering period,15 high-quality cultivars have been selected and 66 cross combinations were obtained.This study provide a theoretical basis for the cultivation of fine strains.(1)63 cultivars' S-genotypes has been identified as following:'Xinjiangyihao'S17/S8, 'Kongxinli'S11/S10,'Liheyihao'S11/Se,'Zhushali'S11/S8,'Batangli'S10/S11,'Pingdingxiang'Sk/ Se,'Daqingke'S10/S8,'Buxiangli'Sc/Se,'Qingzhouli'S41/Sc,'Lianpingsanhuali'S10/S6,'Zaoli'S 10/S15,'Lihesihao'S11/Se,'Hengdaohezidahongli'S17/S10,'Pingguoli'S15/S16,'Ziyunjixueli'S17 /S11/Mili'Sc/Se,'Furongli'Si/Se,'Zaoshuli'S9/S18,'Jinshali'S18/S10,'Dahuangli'S10/Sg,'Qiuho ngli'Sb/Sg,'Suilenghongli'S10/Sg,'Zaohuangli'S10/Sg,'Kangshili'S10/Se,'Fenghuali'S7/Sb,'G uali'S32/S11,'Lizimei'S10/S22,'Huizili'S17/S32,'Zaoshuhonglizhen'S18/S9,'Kongquedanshishe ng'S10/S8,Shuijingli'Sc/Se,'Wumingli'S17/S8,'Guangdonghongxinli'S10/S14/Wuxiangli'Sk/ Se,'Yelicang'S3/S35,'Naimanwanshu'S22/S11,'Badou'S11/S26,'Youyiyinbai'S12a/S26,'Kezike ximixi'S14a/S9,'Tuxiangbai'S22/S4a,'Zhejiangxing'S17/S37,'Heiyexing'S8/S27,'Jinxidahongxi ng'S16/S22,'Jiubalingsan'S12/S3,'Jinkui'S22/S17,'Maihuangerhao'S10/S22,'Yinxiangbai'S23/S3 6,'Longkenwuhao'S39/S8,'Guanyeliansihao'S24/S38,'Lanzhoudajiexing'S11/S22a,'Shajinhong 'S16/S22b,'Weiwang'Sh/Se,'Weidi'S1/Sb,'Weixin'S2/Se,'Weihou'Sh/Se,'Konglongdan'S1/Sb,' Fengweihuanghou'S1/Sb,'Fengweimeigui'Sh/Se,'Zaobaihuali'S10/Sx,'Wanshuhuanglizhen' S8/Sx,'Wuy ueli'S10/Sx,'Guangdonghongli'S23/Sx,'Xi anhebao'S40/Sx. (2)15 new S-RNase gene from pistil and 3 new SFB gene from pollen have been isolation.S41,S23,S12a,S36,S22b,S22a,S39,S38,S35,S40,S14a,S32,S37,S4a,S23-RNase gene from pistil have been isolation from'Qingzhouli','Guangdonghongli','Youyiyinbai',Yinxiangbai', 'Shajinhong','Lanzhoudajiexing','Longkenwuhao','Guanyeliansihao',Yelicang', 'Xianhebao',Kezikeximixi','Guali',Zhejiangxing','Tuxiangbai',SFB32,SFB37,SFB22 gene from pollen have been isolation from'Guali','Zhejiangxing','Tuxiangbai'(3)15 high-quality cultivars have been selected and 66 cross combinations were obtained.On the basis of cultivars' S-genotypes and mean fruit weight and fruit quality,'Weixin','Weiwang','Weihou','Weidi','Fengweihuanghou','Fengweimeigui','Kon glongdan','Pingdingxiang','Kezikeximixi','Lanzhoudajiexing','Fanghuali','Yinxiangbai' 'Mili','Kangshi','Batangli'were selected from 63 cultivars.Conprehensive flowering period,66 cross combinations were obtained from 210 cross combinations.This study will provide theoretical guidance for the production and practice.