| The Tung oil tree has the unique characteristic and has the potential to be the model plant in non-timber forest. In this experiment the duinian which has early-flowering characteristic was applied as the plant materials. The plant were treated with 6-BA, ABA and GA in different concentration level. The purpose of the experiment is to research the effect of 6-BA, ABA and GA on the endogenous hormones of the Tung oil tree, the main nutritious elements, and the differentiation of flower buds. So as to understand the Tung oil tree's physiological and biochemical principium, show the internal relations and reciprocity between endogenous hormone and nourishment at the process of flower bud differentiation, and provide the evidence of florescence regulate and culture management. The main results of the thesis are as below:1,Using 6-BA, ABA and GA could promote the flower bud formation of Tung oil tree. The treatment B2 (ABA 100mg/L) and A1 (6-BA 25mg/L) have the best simulative effect. The flower bud differentiation achieve to 88.86% and 86.83%, which have improved to 41.28% and 39.26% compare that of the contrast. It has less effect of the flowering bud formation by using A3 (6-BA 100mg/L) and B3 (ABA 200mg/L). Using GA could reduce the flowering bud formation, and C3 (GA 200mg/L) has remarkable inhibiting effect. Square difference analysis shows that kinds of treatment ways have reached significant level except A3 and B3.2,In the early period of physiological differentiation and morphological differentiation, the treatment 6-BA and ABA could make the content of soluble total sugar and soluble starch turn uptrend. In the ending period of physiological differentiation and morphological differentiation, the content of soluble total sugar and soluble starch turned downtrend. Although the leaves total sugar and starch's transformation of treatment GA was similar with treatment 6-BA and ABA, but the content normally was same or less than the contrast. It indicates that treatment of endogenous hormones reinforced the transportation and distribution ability of leaves total sugar. The C/N ratio was also adjusted. So it could accelerate the flower bud differentiation.3,The soluble protein content turned the trend up-and down orderly. It shows that the accumulation of protein is necessary for flower bud differentiation. The treatment GA promote the content of soluble protein content.4,In the period of physiological differentiation, the treatments 6-BA and ABA could decrease the content of IAA and GA in leaves obviously, and increase the content of ABA and ZR of plant remarkable. With the contrary effect, the treatment GA could increase the content of IAA and GAand decrease the content of ABA and ZR of plant obviously. For overview, in the period of physiological differentiation, the level of GA has un-remarkable movement. It has the lowest level of the four hormones which tested. In the early period of physiological differentiation, the content of IAA, GA, ABA and ZR could increase to a flood tide. In the middle and ending period of morphological differentiation, the content of GA and IAA were decreased by fluctuations, and the content of ABA and ZR were decreased continuously. In the early period of physiological differentiation, the content of IAA, GA, ZR and ABA increased together. It is beneficial for the balancing of ABA/IAA,ZR/GA, and it is helpful to improve the flower bud differentiation and the flower quality.5,In the period of flower bud differentiation, treatments 6-BA and ABA could increase the ratio of ZR/GA, ZR/IAA, ABA/IAA and ABA/GA remarkably. It has opposite effect for treatment GA which could restrain the improvement of these four ratios. Among those treatments, treatment B2, A1, A2 and B1 were more effective than treatment A3 and B3. It shows that the level of ABA and ZR are the key facts for the improvement of flower bud differentiation and flower quality.
|
PDF Full Text Request