Research On Several Key Techniques Of Quality Assessment And Quality Control Of Radix Actinidiae Macrospermae

Actinidia macrosperma C. F. Liang (Cat Ginseng) is one of the most frequently applied traditional Chinese medicine (TCM) for antitumor therapy, especially against alimentary canal cancers in East China. In order to exploit this resource in China with quality assessment and quality control, the following studies were carried out.1) HPLC method was established for quantify determine of TCM Cat Ginseng determining the ursolic acid, asiatic acid and 2a,3a-dihydroxy-urs-12,18-dien-28-oic acid in rude medical material of Cat Ginseng for the first time, which were successf-ully separated on an Aglient HC-C18 column (250 mm×4.6 mm,5μm) at 35℃. The mobile phase was acetonitrile-water employing a gradient elution at a flow rate of 0.8 ml/min, and the detection wavelength was set at 210 nm. All results showed that the method of HPLC fingerprinting analysis was good and satisfactory. Simultaneously, we found the quality of Cat Ginseng not only related to absolute content of the reference components but also related to their relative proportions.2) Through analysis and comparison of fingerprint, reference components and anti-tumor activity in vitro from different Actinidia species and medicinal parts, we found between A.macrosperma and A. valvata had significant differences. Therefore, we believe that the original plant of Cat Ginseng should only A. macrosperma, not including A. valvata. Moreover, the three reference components of stems and roots from A.macrosperma were not significantly different, so the two parts could be used as medicinal parts.3) Through analysis and comparison of different types of Cat Ginseng, we found the absolute content of the three reference components from different regions had small differences, but existed significant differences in their relative proportions. Taking into account pharmacological experiments, preliminarily, we suggested that the best quality medicines produced in Fuyang (relative proportions of asiatic acid, ursolic acid and 2a,3α-dihydroxy- urs- 12,18- en- 28-oic acid were 12:31:57). The content and proportions of three reference components fluctuated with the month and phenology, combining with the view of pharmacological experiments and traditional excavation, we believe that 11-12 month period is the best harvest period for Cat Ginseng. Although displayed only small difference in the reference components of different cultivation, considering yield, scaffolding was more appropriate for Cat Ginseng.4) The relationship between the content of reference components and physical and chemical properties of soil from different tested areas was not significant, so there was no strict requirements for the soil of cultivation.In the present study, an efficient strategy is proposed for quality control of A.macrosperma as raw materials in the pharmaceutical and natural product industries, especially, solved the problem of the original plant and medicinal parts of A.macrosperma which troubled people for a long time, which forms a foundation for further exploitation and use of A.macrosperma resource.