Study On Transformation Of ZmERD4 Gene Into Maize Mediated By Agrobacterium Tumefaciens

Maize is one of the most important crops and poultry diets and fuel in the world. Drought is one of the most important limiting factors during plant growth and development, and maize is more sensitive to water. To further investigate the function of ZmERD4 gene, this study has constructed ZmERD4 gene over-expression vector, RNAi vector and drought stress induced vector, and through Agrobacterium-mediated transformation of maize immature embryos, embryogenic callus and shoot meristem to obtain research materials of ZmERD4 gene, and expect to be drought resistance transgenic maize. In addition, by observing the regeneration callus cells shape of different types of callus squash under the microscope, and using Agrobacterium-mediated transformation of maize immature embryos, this study has selected 45 inbred and 13 genotypes among them can regenerate seedlings, namely:10MZ54,6AT,10MZ255, Chenghai 701 female parent, HL6, B73, X29010-3102, C103, Liao 5110, A×B, Zong 3 and 2041 (unknown species). Using the foregoing immature embryo and A×B induced callus as the receptor materials,149 regenerated plants have be obtained. By using the genomic DNA of 149 To generations as template, PCR amplified 55 strains containing the Bar gene plant with BarF and BarR primers, PCR positive rate was 36.9%; using specific primers ERD4-1634S and NOSUTR-141A for PCR amplification test,42 strains were detected positive plants, PCR positive rate is 28.2%; By Zheng 58 shoot meristem as a receptor material,32 regenerated plants has been obtained, Bar gene positive rate is 15.6%; interesting gene positive rate is 6.3%. These results show that ZmERD4 gene has been introducted into maize genome, which obtain the transgenic maize with ZmERD4.