| The components and content of HMW-GS in wheat grains affect size of glutenin particleand thus flour quality. They are the important traits of wheat quality which reflect strengthsand weaknesses of wheat flour. In 2008-2010 growing seasons, three wheat cultivars withdifferent end-use qualities were used to study the effects of irrigation-nitrogen interaction onrelative content of each HMW-GS, content of total HMW-GS and GMP size distribution inthe experimental farm of Shandong agricultural university by RP-HPLC and laser granularityanalysis instrument .The principle results are as following:1 The effects of water-nitrogen interaction on nitrogen accumulation, transferring in wheatFlowering, leaf nitrogen content of three wheat varieties are in response to nitrogenfertilizer in different water treatment are as follows: in response to nitrogen fertilizer in theirrigation conditions more sensitively than its in the non-irrigation conditions; comparisonbetween different varieties, weak wheat is in response to nitrogen fertilizer more sensitivelythan the others.At maturity, N content of the various organs of GC8901 in different water and nitrogentreatments showed the highest grain nitrogen content, spike axis+glume was the lowest.Nitrogen content of TS23 and SN1391 in grain was expressed as the highest, followed byupper leaves, stem+sheath minimum.Compare with no irrigation, irrigation Twas conduciveto Tthe nitrogen TaccumulationT of wheat. In no irrigation, it is conducive to transfer N fromnutrition organs to grain of wheat at anthesis, so that it has distribution of a higher proportionof nitrogen in the grain. Tthe nitrogen contents of Tboth Tmedium and weak wheat were enhancedby increased nitrogen application in no irrigation.Irrigation can promote nitrogen to transfer from nutrition organs to grain of GC8901 andSN1391more effectively, to obtain a high translocation and contribution efficiency to grainsof nitrogen accumulated in vegetative organs at anthesis, TS23 is opposite. The transfer andthe contribution to grain of three wheat at anthesis response to nitrogen fertilizer by waterfactors controlled. In both water conditions, nitrogen was transferred from vegetative organs to the grain of GC8901 by using proper nitrogen fertilization, and to obtain a highercontribution rate; irrigation conditions, no nitrogen can increase the transferring rate and thecontribution rate of TS23, rather than irrigation conditions more conducive to increase thetransferring rate and the contribution rate by using nitrgon fertilizer. no irrigation conditions,the transferring rate and the contribution rate of SN1391 were increased by applying lownitrogen fertilizer; irrigation condition, these were increased by applying high nitrogenfertilizer.2 The effects of water-nitrogen interaction on nitrogen use efficiency and yield of wheatIn 3 wheat varieties, the NUE and HI under rainfed trial were higher than that underirrigated trial. The results suggested that the rainfed condition could promote the NUE and HI.The N production efficiency in GC8901 and TS23 manifestations were as follows: irrigatedtrial > rainfed trial, while that in SN1391 under rainfed trial was higher than irrigated trial.The results suggested that there were genotypic differences in the effect of water treatment onN production efficiency.Moderate water stress can improve nitrogen use efficiency of wheat and harvest index,while irrigation can promote nitrogen production efficiency of wheat, and also promote grainyield, grain number, grain weight, grain protein yield of strong and medium gluten wheatincreased, and significantly increase the average protein content of strong gluten wheat, whilethere is little effects of water on protein content of medium and weak wheat. TThe grain yieldof 3 wheat varieties all increased with nitrogen fertilizer increased to N2 level, to furtherimprove the nitrogen fertilizer level then it reduced in the different water treatments.T On thewhole,irrigation conditions, the medium nitrogen level can coordinate the relationship of theproduction of wheat grain yield and nitrogen production efficiency, nitrogen use efficiencyand harvest index, to obtain high yield and efficiency.3 The effects of water-nitrogen interaction on Tthe contents of HMW-GS and the proportion ofthe size particles of GMPHMW-GS composition of wheat is controlled by genotypes, the proportion of a singlesubunit (except Bx7, By8 subunit) of three wheat varieties to the total content of HMW-GS inthe different water and nitrogen treatment keep stability in some extent. Under the irrigationconditions, increased rate of nitrogen fertilizer promoted the TaccumulationT of both THMW-GSand LMW-GS, and raised the proportion of the larger particle Tof TGMP and the GMP contentin grain of wheat cultivars GC8901 and TS23. Meanwhile, the accumulation of both HMW--GS and LMW--GS in grain of weak wheat cultivar SNl391 was reduced due to theapplication of nitrogen fertilizer,which was conducive to the formation of large size particlesin GMP As compared with the irrigation conditions,increase the rate of nitrogen fertilizerunder no irrigation conditions resulted in the increased contents of both HMW-GS andLMW-GS in grains and the increased proportion of large size particles in GMP in weak wheatcultivar SNl3914 Correlation analysis ofyield and qualityThe analysis of correlation showed that yield was significantly positively correlated withnitrogen content,height and negatively correlated to 1000-grain weight,K,Rmean,respectively,and negatively correlation with Rmax Yield mad ear length,the amount of nitrogen fertilizerhad no significant correlationThe effect ofH,L on the content ofGMP was similar,madthe effect ofx-type subunRson GMP was greater than Y-type subunits The correlation of GMP content and a singlesubunit showed that except for Bx7and Dyl0,the other subunits mad GMP were significmatlycorrelated,while the correlation ofGMP and Dx2+Dyl2 was more significantly than others...
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