Quick Propagation Techniques Of Sophora Japonica Golden Stem And Other Color Trees

1,The hormone matching of media had little influence on seed germination.6-BA could promote the production of callus.The most suitable induction media was MS+2.0 mg/L6-BA+0.2 mg/LNAA+0.5 mg/LKT for somatic embryo,and MS+1.0 mg/LBA+0.1 mg/LNAA for bud.The rooting media was 1/2 MS+0.3 mg/L NAA. Active carbon went against the rooting culture of Sophora japonica Golden Stem.The seed germination rate relates to seed quality of S.japonica and has little correlation with the kinds of media.Cotyledon was most suitable for inducing callus.2,The germination rate of Lagerstroemia indica'Summer on medium with the hor-mone at some chroma.It was obviously higher than that in medium without the hormone.The medium for inducing the L. indicatube seedlings was MS+6-BA1.0 mg/L+NAA0.01 mg/L.The budding-rate is more longer than the growth state of bud varied obviously with the change of 6-BA,NAA and KT chroma. The optimum medium for proliferation was MS+6-BA2.0mg/L+KT1.0mg/L+NAA0.01~0.05 mg/LMS+NAA0.5 mg/L.The optimum medium for rooting was MS+NAA0.5 mg/L, on which the tube seedlings grown strongly with more and thicker roots. The chroma of hormone such as 6-BA, NAA and KT had great effect on forming and rooting of L. Indica clustered shoots.3,MS+BA0.6mg/L is the effective inducing medium.Medium contains MS+BA0.5mg/L+IAA0.2mg/L+GA0.5mg/L is the best differentiation medium for buds, 1/2MS+IAA0.2mg/L is the best radiation medium and sand of cinder is the suitable medium for transplanting or cuttage. Cuttage increases the efficiency of propagation.4,MS no hormone was the suitable basal medium at the beginning of the cultiuation. When the coneentration of 6-BA was 1.0mg/L ,explants show symptoms of hrmone poisoning and leaf necrosi. SegmentsofePieotyl, 6-BA0.1mg/L+NAA0.2mg/L was the suitable basal medium.When the eoneentration range of 6-BA was 0.1~0.5mg/L, micropropagation was suitable. When the coneentration of 6-BA was more than 0.5mg/L,the leaf was rankle,even vitrification. The coneentration range of 6-BA of 0.1-0.2mg/L was the most suitable. Root indueion , 1/2MS+NAA0.3mg/L was the suitable basal medium.