Study On The Effect Of Cottonseed Hull-based Diet On Drinking, Urination And Water Metabolism In Sheep

Objective: To study the effect of cottonseed hulls on Drinking, Urination and water metabolism in sheep.Diuretics can be better used and urolithiasis can be reduced in sheep by the experiment provided reference.Provide reference for reducing urolithiasis by chooseing an appropriate diuretic.Method:.This experiment components three trials.In trialâ… ,analyzed the effect of different cottonseed hull contents on drinking water,urinary output and other parameters.In trialâ…¡, studied the effects on water metabolism by feeding the sheep with cottonseed hulls and the reasons.In trialâ…¢,choose a diuretic appropriately to reduce the effect of cottonseed hulls on urination.Results: Trialâ… : The drinking of tested sheep in the 4th declined by 13.11% (P<0.01) compared with the 1st period,The urination of tested sheep in the 4th declined by 63.02% (P<0.01) compared with the 1st period. The fresh fecal content in the 4th period increased by 42.86% compared with that of 1st period sheep,the difference was extremely significant(P<0.01). By statistical results, the dry matter content in urination of 4th period sheep was less than 1st period by 46.38%.The water in exerement was increased gradulally,that of 4th period sheep was increased by 0.32kg/d compared with that of 1st period sheep, the difference was extremely significant(P<0.01). Water discharged from the tested sheep had little change in early three periods,but decreased in 4th period,which has the biggest difference with 1st period,but was not signigicant (P>0.05).Trialâ…¡: The content of antidiuretic hormone of the sheep in the fourth period was 2.29 times than the first period with an extremely significant difference (P<0.01). The content of adrenocorticotropic hormone of the sheep in the fourth period increased by 47.02% compared with that of 1st period sheep,the difference was extremely significant(P<0.01). The content of corticosterone of the sheep in the fourth period decreased by 54.34% compared with that of 1st period sheep,the difference was extremely significant(P<0.01). However, there were no significant effects (P>0.05) on the contents of the acetylcholine, adrenaline, noradrenaline, aldosterone, rennin, angiotensinâ…¡, growth hormone, calcitonin, atrial natriuretic peptide. In serum of the sheep, the concentrations of Na⁺, Cl^-, K⁺, P increased after the cottonseed hulls were fed (P<0.05, P<0.01), while the concentration of Mg²⁺ decreased, where it's concentration of the first period decreased by 28.83% compared with that of the fourth period, with extremely significant difference (P<0.01). There was no significant change (P> 0.05) in the concentration of Ca²⁺. In the urine of fed sheep, the P content in the fourth period increased 21.67% compared to the first one in which the difference was extremely significant (P<0.01). The contents of Na⁺, Cl^-, K⁺, Ca²⁺ and Mg²⁺ all decreased (P<0.01).Trialâ…¢: After 350mg GY^-01 was supplemented, the water drinking amount of each sheep had an increase of 60.14% compared with that of the sheep in the control period and the urination volume was 2.67 times of that of the latter, and the differences were both significant (P <0.01). The antidiuretic hormone declined by 32.59% compared with that of the sheep in the control period with the significant difference (P <0.05). The adrenocorticotropic hormone content increased by 81.93% compared with that of the sheep in the control period and the difference was significant (P <0.01). An increase of 28.04% (P <0.05) and 39.39% (P <0.01) was found in the contents of serum potassium and phosphorus respectively compared with that of sheep in the control period.Conclusions: Trialâ… : Duing the four periods,the level of drinking of the sheep had little change with the increasing of the feeding contents of cottonseed hull.The urination of sheep declined extremely.The water in exerement was increased.The dry matter content in nutrnation was decreased.Water discharged had little change.Trialâ…¡: In the serum of sheep fed with cotton seed hulls, the antidiuretic hormone and adrenocorticotropic hormone levels rised, while the corticosterone level decreased. The contents of Na⁺,Cl^-,K⁺and P increased while that of Mg²⁺ decreased, and Ca²⁺ showed no significant change in serum. In urine, P content increased, while the contents of Na⁺,Cl^-,K⁺, Ca²⁺, Mg²⁺ decreased, resulting in extremely significant reduction in sheep urinary output.To conclude,water metabolism could be influenced by feeding cottonseed hulls through adjust antidiuretic hormone, adrenocorticotropic hormone and corticosterone hormone.Trialâ…¢:Suggest to add 200mg GY^-01 in daily feeding of each sheep could increase the effectiveness of diuresis by feeding cottonseed hulls.