Separation And Purification Of Saponins And Polysaccharides From Jiuhua Mountains Polygonatum Cyrtonema Hua.

To get high bioactivity and high purity bioactive substances from natural plants is the study hotspot in biology and medicine fields. So far, the bioactive substances from Jiuhua mountain Polygonatum cyrtonema Hua. are not been studied, even though it is edible and medicinal with excellent qualities and mentioned in the same breath with Changbaishan Gensing. The polysaccharide and saponin from Jiuhua mountain Polygonatum cyrtonema Hua. were separated and purified by a continuous process, and the bacteriostatic activity of the polysaccharide also studied.The results were as follows:(1) The orthogonal experiment showed that an ultrasonic extraction of the best conditions for saponins: ethanol concentration 75%, extracting time 50min, material/liquid ratio 1:20 and repeat extraction 2 times. The factors affecting extraction ratio were material/liquid ratio >ethanol concentration >extraction times >extracting time.(2) The best way to purify saponins was first to purify with water saturation normal butanol and then to reoccupy the purification with macroporous resin XAD4, to achieve the purity of 96.33%.(3) It was calculated that the average purification of every 100g of Polygonatum cyrtonema Hua. can be extracted to 4.17 g. saponins with purity of 96.31%.(4) Polysaccharide extracting results showed that: it was feasible to first extract saponins but the results show no obvious difference. The best approach to extract polysaccharide was ultrasonic with cellulose enzyme. The highest rate for gross polysaccharide was 20.6%.(5) There is a kind of pure Polygonatum cyrtonema Hua. polysaccharide, its molecular weight, 8912, its composition, fructose : glucose = 8.7:1.(6) The best way for purification of polysaccharides was Sephadex G- 50 gel filtering method, the purity of 96.80% reached.(7) Jiuhua mountain Polygonatum cyrtonema Hua. polysaccharide had certain antibacterial activity, especially for Escherichi Coli, Salmonella. Paratyphi, Staphy lococcus cremoris and so on.This research showed that Jiuhua mountain Polygonatum cyrtonema Hua. contains rich and various kinds of bioactive substances, to study these substances will be helpful for promoting the economic value and social effects.