Studies On Cell Microfilaments And Volatile Metabolies Of Rice Inoculated With Magnaporthe Grisea

In plant-pathogen interaction, the infected host cells initiated a cascade of resistance-related events in order to prevent pathogen infection. After infected with the pathogen, not only the host cell cytoskeleton, but also the host's metabolic pathways and metabolites changed. This research presented a detailed investigation of Magnaporthe grisea spore germination, appressorium formation and it's successful infection of the host cells after inoculated in the rice leaf sheath, and the host cell's microfilament re-organization and the changes of volatile metabolites of the rice leaf sheath during the early stages of disease development and showed difference between the compatible and incompatible interaction. The main results were as follows:1. Treatments of spore suspension with the microfilament inhibitor cytochalasin D (CD) indicated that 1-20ug/ml CD treatment had no effects on spore germination and appressorium formation of M. grisea strain YOU 2. There is no difference in the spore germination, appressorium formation and successful infection of host cells of the M. grisea in resistant rice P816 and susceptible rice SE21S.3. Inoculated with M. grisea, the infected host cell microfilament was damaged, but the extent of damage of P816 was less than that in SEE21S.4. Many of volatile metabolites of P816 inoculated with M. grisea were different from that with sterile water. The similar results were found in the volatile metabolites of SE21S. The volatile metabolites were also different between P816 and SE21S.