Study On Low-dose Pesticides To Relative Activity Of Paddyfield Spiders And The Structure And Functions Of The Spiders' Midgut Cells

Influence of Low-dose Pesticides to Relative Activity of Paddyfield SpidersThis research reports for the first time that the low-dose chemical pesticides can strengthen relative activity and insect-control power of spiders, and have an initial research and discussion about the reason why their relative activity are strengthened. It is concluded that the proportion of the most suitable density of Dimehypo solution is 14:5000 and 12:5000, which can bring into play the potential of insects-control power of Pirata subpiraticus and Pardosa pseudoannulata, and the proportion of the most suitable density of Methamidophos and D-allethrin is 2:5000 and 1:20, respectively. The creature-number result of spider controlling pest was stintly measured.Effect of low-dose pesticide on the midgut cells and the digestive enzyme activities of the wolf spider, Pardosa pseudoannulataThe effects of pesticide on midgut cells and digestive enzyme activities of the wolf spider, Pardosa pseudoannulata were determined by means of electronmicroscopy and biochemical analysis. The results of biochemical analysis showed the activities of protease and lipase of the wolf spider midgut had significant changes under the fittest low-dose pesticide compared with those of the normal. Under the fittest low-dose pesticide, the activities of protease and lipase of the wolf spider midgut is significantly increased. It was observed that the midgut cells and their organelles of the wolf spider under the different density pesticide had significant changes. Under the fittest low-dose pesticide, the microvilli of the column cell were ordered and the number of microvilli was increased markedly, and the number of mitochondria and rough endoplasmic reticulum were also enlarged. Under the higher dose pesticide, the results were opposite.