Study On The Effects Of Fermentation Time On Constituents Of The Fermentation Fluid Of Organin Materials And Young Apple Tree Under Replant

The experiment was investigated in in the fruit roots laboratory of college of horticultural science and Hongmiao village of Tai'an city (an experimental base) during 2009～2011.The test used Fuji biennial grafted apple seedlings as experimental materials. Under planting in pots, the change of main contents in different anaerobic fermentation time at different season and the effect of fermented fluid from organic materials on the biomass of apple seedlings, the pHysiological index of root and impact of soil environment .The main results are below:1. According to the different fermentation time in different seasons, the content of fermentation fluid changed obviously, above them, especially the change of the compositions such as amino acid, hormone, vitamin b and organic acids fermented 60 d in spring, 45 d in summer and 45 d in autumn was evident.2. All the fluids with anaerobic fermentation at different time in different seasons promoted the height, shoot diameter, fresh weight and dry weight of apple seedlings. Among them, T3 in spring increased more compared to T1 and T2, in detail, 15.98 increase in stem height, 0.022 increase in ground diameter, 31.7 increase in fresh weight and 25.78 increase in dry weight compared to controls, respectively. And the increase amplitudes were 4.3% and 28%, 47%, 33%, respectively. T1, T2 and T3 in summer and autumn enhanced markedly the height, shoot diameter, fresh weight and dry weight of apple seedlings in contrast to their respective controls. Thereinto, the effect of T2 and T3 was almost equal, and the both were greater than T1.3. All the fluids with anaerobic fermentation from organic materials improved the respiratory rate and the net pHotosynthesis rate of the function leaves. Moreover, the treatments were higher than the controls. However, different treatments were not significant among them. In addition, the net pHotosynthesis rate of T2 was greatest than T1 and T3, 20.16% greater than its control.4. The MDA level in root treated by fermented fluid from organic materials were lower than controls, but the effect of different anaerobic fermented fluids on root was different. T3 was the most significant in spring, only 35.3% of its control level. The MDA levels of T2 and T3 decreased greatly, 42.8%,35.7%. The difference of T2 and T3 in autumn was not evident, but appreciably lower than their controls, 42.8%,35.7% of their controls, respectively.5. Compared with the contrast, after applying the fluids with fermentation from organic materials, the activities of soil urea enzyme, catalase enzyme, sucrose enzymes and neutral pHospHorylating enzyme increased significantly. The activity of urea enzyme in different seasons among different treatments varied, the levels of T3 were greatest in spring and autumn, 2.26-fold and 2.29-fold more than its controls. T2 was the highest in summer and 2.17-fold greater than its control. The activity of catalase enzyme among different treatments slightly varied in different seasons, but the differences were not obvious. The activity of sucrose enzymes in T2 was the greatest during assay in spring, summer and autumn, and was 1.9,1.8 and 1.43-fold greater than its repective season control. The increasing activity of neutral pHospHorylating enzyme in T1, T2 and T2 in spring, summer and autumn was remarkable, and was 2.8, 1.87 and 3-fold greater than its repective season control.6. The correlation analysis results indicate:the composition of fermented fluid from organic materials with biomass closly are 6BA,VB12 and Malic acid its coefficient of correlation with dry weight respectily achieved 0.65,0.81and 0.53.