Immunization Of Recombinant Lactococcus Lactis Expressing Transmissible Gastroenteritis Virus S Gene To Mouse And Newborn Piglets

Transmissible gastroenteritis virus(TGEV) belong to Coronavirus,it is the etiological of TGE,which is a highly contagious enteric disease in pigs causing graveness diarrhea,dehydration vomit,with developing of TGE,it poses hazardous in same with Classical Swine Fever and Porcine Reproductive and Respiratory Syndrome.It is good for prevention beforehand TGE to mucous membrane immunifaction.This immuniton method can cause intestines mucous membrane to secrete SIgA so as to achieve to prevent beforehand TGE.In the world,it is a lot of regarding to TGE vaccinal research,the TGE vacinne exist different shortcoming, so it is tough problem for researchist to search for TGE genetically engineering vaccine.According to studies on TGEV,it belong to positive-strand, single-stranded RNA virus,its genome which make up of Constitutive Protein and Nonstructural Protein. S (spike protein) is one of Constitutive Protein,it is encoded TGEV part protection gene that locates on the surface of virion.The spike protein has favourable antigenicity and it is only one protein that induces generating neutralization antibody.The recombinant lactococcus lactis named PNZ8149-NZ3900 with S gene of Transmissible Gastroenteritis Virus were cultured and induced by Nisin, then orallied immunization to newborn piglets and BALB/C mouse. The lymphocyte proliferation stimulating index and IgG in the serum of newborn piglets and mouse, SIgA in the intestine mucous membrane of newborn piglets and mouse were measured by MTT and indirect ELISA respectively. The results showed that the lymphocyte proliferation stimulating index of test animals immunized with recombinant bacteria was significant higher than that of control animals, IgG in the serum of immunized newborn piglets and mouse with recombinant bacteria and SIgA in the excrement and urine immunized of the newborn piglets and mouse with recombinant bacteria were higher than those of control animals. Illustrating recombinant lactobacillus possessed high-blooded immunogenicity and could induced animals to produce satisfactory mucous membrane immune response and eneral immunity.