| A 42-day feeding trial was conducted to study the effect of transgenic Glyphosate-tolerant soybeans on the intestinal health in Arbor Acres broilers, and evaluated the feed value of it, afford the experiment support for feeding safety evaluation of the transgenic Glyphosate-tolerant soybeans.Three hundred and sixty day old broilers were randomly divided into two groups, they were fed the diet containing transgenic Glyphosate-tolerant soybean meal and conventional soybean meal respectively. They were slaughtered in 21d and 42d for sampling. The experiment has comparatively determined the general nutrient, the amino acid, the fatty acid, the mineral element, and the anti-nutrient factors of the transgenic soybean meal and the conventional soybean meal; determined the effect of the transgenic soybean meal on the growth performance and the carcass parameters in broilers.; evaluated the effect of the transgenic soybean meal on the intestinal health in the broilers, it has studied from the intestinal mucosal immunity and the balance of the intestinal microflora angles. The result showed:(1) There's some difference between the transgenic soybean meal and conventional soybean meal in nutrition composition; 0-21d, the ADG and F/G radio weren't significant different between the two groups (P>0.05), but the ADFI of the TSM group was significant high than the CSM group (p<0.01); 22-42d, the ADG of the TSM group was significant high than CSM group (P<0.05), but the ADFI and F/G radio were not significant different between the two groups(P>0.05). 42 d, the eviscerated weight of the CSM group were significant high than the TSM group(P<0.05), the other carcass parameter weren't significant different(P>0.05).(2) The numbers of iIEL in different parts of intestine were not significant different between the two groups (P>0.05); The sIgA content in broiler's jejunum chyme was not significant different between the two groups (P>0.05), and it's significant different between the two phases(P<0.01); The content of the Th1/Th2 cytokines in broiler's jejunum organization was not significant different in two phases between the two groups (P>0.05), along with the growth of the age , the Th1/Th2 balance was shifted to Th2.(3) The band number of PCR-DGGE fingerprint of the microbe in ileum and cecum were not significant different between the two groups (P>0.05). Similarity of the total ileum and cecum microflora between the two groups was about 62% and 58% respectively, in the same feeding condition, there's a biggish individual differences in the intestine microbial species of broilers. The PCR-DGGE electrophoresis pattern bands of ileum microbe have divided into two groups because of the different of feed, but haven't changed in cecum microbe. Gene sequence analysis showed, 11 clones showed their similarities with database sequences 100%, while the other four clones present similarity over 93%. In these sequences, have 4 sequences are identified as known bacteria, the rest are similar to those unidentified bacteria, there have two sequences are common in both ileum and cecum.In conclusion, transgenic soybean meal and conventional soybean meal have no"substantial equivalence"in nutrition composition. In the period of the experiment, there were no adverse effects of the transgenic soybean meal on the growth performance, carcass parameters, intestinal mucosal immunity, and the intestinal Microflora of Broilers, it hasn't effect the intestinal health of the broilers.
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