Effects Of Chitosan Oligosaccharide Supplementation On Production Performance,Immune Performance,Serum Antioxidant Activity And Intestinal Development In Broilers

The effect of dietary chitosan oligosaccharide(COS)supplementation on growth performance,slaughter performance,meat quality,immune performance,serum antioxidant activity,intestinal mucosal structure and intestinal flora was assessed by feeding graded levels(0,50,100,150 mg/kg)of COS.300 male Arbor Acres broilers(1-d-old)were assigned randomly to 5 dietary treatment groups(10 birds per pen with 6 pens per treatments).The negative control was the basal diet without any supplement.The positive control was a basal diet supplemented with 50 mg/kg of chlortetracycline.Three levels of COS,50?100?150 mg/kg were added to basal diet in experiment groups.The results showed as follows:1.Effects of Chitosan oligosaccharide Supplementation on growth performance,slaughter performance and meat quality in Broilers: Dietary supplemented with 100 mg/kg of COS significantly improved BW?ADG?F/G of 42-day broilers compared with control group(P<0.05),and there was no difference compared with antibiotic.Dietary supplemented with 100mg/kg of COS significantly improved percentage of half-eviscerated?percentage of eviscerated and percentage of leg muscle compared with control group(P<0.05),the percentage of half-eviscerated and percentage of eviscerated even significantly higher than antibiotic group(P<0.05).Dietary supplemented with 100 mg/kg of COS significantly improved a* value of breast muscle and thigh muscle(P<0.05).The L* value of thigh muscle increased.The drip loss of thigh muscle decreased closed to prominence.2.Effects of Chitosan oligosaccharide Supplementation on immune performance and serum antioxidant activity in Broilers: Dietary supplemented with 100 mg/kg of COS significantly improved IgG and IgM of 21-day broilers compared with control group(P<0.05),and significantly improved thymus index and burse index of 42-day broilers(P<0.05).There was no significant difference in IgG,IgA and IgM at 42 days of age in each treatment group(P>0.05).Dietary supplemented with chitosan oligosaccharide had no significant effect on the antibody titer of Newcastle disease in serum(P>0.05).Dietary supplemented with 100 mg/kg of COS significantly increased total antioxidant capacity(T-AOC),T-SOD activity and glutathione peroxides(GSH-Px)activity of 42-day broilers(P<0.05).Dietary supplemented with 50 mg/kg COS also significantly increased T-SOD activity and GSH-Px activity of 42-day broilers(P<0.05).There were no differences when supplemented with 150 mg/kg of COS.3.Effects of Chitosan oligosaccharide Supplementation on Intestinal mucosal structure and Intestinal flora in Broilers: Dietary supplemented with 100 mg/kg of COS significantly improved villous height(V),villous height/crypt depth(V/C)of jejunum,and significantly decreased crypt depth(C)(P < 0.05).Dietary supplemented with 50 or 100 mg/kg of COS significantly reduced the number of ileal E.coli(P<0.05).The number of Bifidobacteria increased when supplemented 100 mg/kg of COS,and there are no significant influence on Lactobacillus(P>0.05).Conclusion showed as follows:It can be concluded that dietary supplemented COS improved growth performance ? slaughter performance and meat quality by increasing immune performance?antioxidant activity and regulated intestinal mucosal structure and flora of broilers.The optimum dosage of COS is 100 mg/kg,it's effect is better than that of 50 mg/kg chlortetracycline.