The Investigation Of Non-food Plant Resource For Biodiesel And Analysis Of Genetic Diversity Of Xanthium Sibiricum In Shandong Province

Under the background of energy shortage and environmental deterioration around the world, more and more attention has been paid to the development of biomass energy. Biodisel is a bioenergy product which is produced from plant or animal oil. The sustainable supply of its feedstock is a key step limiting its development. Cultivation of biodiesel crops on good soil is not practical in China because the arable land resource is limited. In this context, it will be alternative approach to develop oil plants for biodiesel on marginal land. This study was done in order to find out such oil plant species suitable for Shandong province. More over, Xanthium germplasm resources in Shandong province were collected and analyzed for understanding its genetic diversity, providing a basis for its breeding and improving investigation method of biodiesel plants. The results were obtained as follows:Based on literature review and on the spot survey, 213 nonfood plant species from 63 families were investigated for their habitats, living conditions, the seed setting, phenophase, associated plants, adaptability and so on. 60 nonfood oil plants were preliminarily targeted for further research.130 species'seeds were collected, determined for the oil content, acid value, saponification value, iodine value and fatty acid composition. The oil contents ranged from 1.10%~ 68.47%, in 26.15% plant species'the oil content were higher than 30%; Acid values ranged from 0.32~80.45mg/g, and 1~10 mg/g in 72.3% species; saponification values ranged from 28.70~427.79mg/g, 100 ~ 200 g/100g in 71.54% species; iodine values ranged from 9.42~199. 86 g/100g, less than 120 g/100g in 63.85% species. The main components of oil fatty acid were oleic acid, linoleic acid and linolenic acid. According to on the spot investigation and biodiesel related quality,17 important species were tentatively identified as potential biodiesel feedstock plants suitable for Shandong province, which included Prunus padus L, Prunus davidiana (Carr.) Franch, Prunus armeniaca L, Prunus persica (L.) Batch, Siegesbeckia pubescens Makino, Xanthium sibiricum Patrin ex Widder, Ricinus communis L., Grewia parviflora Bunge, Rhus typhina Nutt, Ziziphus jujuba var.spinosa(Bunge)Hu, Euonymus bungeanus Maxim, Euonymus japonicus Thunb, Dature Stramonium L, Abutilon theophrasti Medic, Ligustrum quihoui Carr, Koelreuteria paniculata Laxm., Oenothera erythrosepala Borb.The oil content was determined and genetic diversity of cocklebur was studied with ISSR molecular markers. The oils contents were significantly different among 29 different accessions from Shandong province, ranged 19.94%~42.31%. The genetic diversity is related with natural segregation condition, it is more diversified in plain terrain than mountain areas. But when clustering with the single plant data, the regional difference in genetic diversity among different accessions didn't show regional characteristic, which showed there exists frequently genetic exchange among different regions. The research on the genetic diversity of Xanthium is important for further study of non food biodiesel plants.