Genetic Relationship Analysis And Identify Breeding Of Hybrid Progenies And Hybrid Parents Of Camellia Oleifera

Modern breeding objectives and requirements of Camellia is a new breakthrough on production in order to change the status of Camellia low current low yield.by hybridization, match excellent hybrid parents, and configure strong hybridized combination,using molecular markers to obtain genetic distance to predict heterosis for the cross breeding to provide powerful analytical tools and theoretical basis. This experiment through ISSR,SRAP two molecular makers to analyze Genetic relationship of 31 samples of Camellia,using molecular markers to obtain genetic relationship between the 31 samples of Camellia.Using the SRAP molecular maker to identify the authenticity of the Camellia parents and their hybrid offspring, using two molecular markers to obtain the genetic distance.Using the methods of the quantitative character ,(including combining ability analysis,hybrid vigor analysis,hybridization survival ratio analysis)to predict the hybrid vigor , match excellent hybrid parents and configure hybridized combination, and provide a theoretical basis for the cross breeding,the main results were as follows:1,In this study , analyze the genetic distance through the ISSR and SRAP two molecular markers, we can find that: 41-4 has the characteristic of paternal inheritance, 27-5,46-2,51-2,56-2has the characteristic of maternal inheritance. Among the hybridized combination by the complete diallel cross, most hybridized combination have partial maternal characteristic, using the 41-4 as the male parent of hybrid crosses, their hybrid offspring are likely to have the paternal inheritance.2,Among the five excellent Camellia, 27-5 and 46-2 have the closest genetic relationship, Compared with the other three plus trees, they have farther genetic distances. the order of hybrid vigor is 27-5 > 46-2 > 51-2 > 41-4 > 56-2. If using 27-5 hybridized with the other three trees has a larger possible hybrid vigor. Similarly If using Min-62 hybridized with the other three trees has a larger possible hybrid vigor.3,Using 17 pairs of SRAP primers and SRAP molecular maker to to identify the authenticity of the Camellia parents and their hybrid offsprings, Identification results indicate that 19 hybridized combination were hybridized successfully and all hybrid offspring are really hybrids.4,Using the methods of the quantitative charater,(including combining ability analysis,hybrid vigor analysis,hybridization survival ratio analysis),combining hybrid offspring seedling stage saplings characters of early selection method, in order to match excellent hybrid parents and configure hybridized combination, the results of the study indicate that: among the five excellent Camellia, no matter in improving camellia fruit quality, or in improving seedling stage growth seedling biomass and hybrid offspring affinity heterosis prediction, 51-2 and 56-2 is suitable for the hybrid female parent and the 27-5 ,41-4 ,46-2 is suitable for hybrid male parent,and the suitable hybrid maternal and paternal hybrid also a good choice to obtain ideal hybridized combinations.Through this study we can conclude that it's convenience for Camellia to use the methods of molecular markers and quantitative trait analysis to match excellent Camellia hybrid parents and configure hybridized combination,which also can Reduce the human, material and time consumption, as well as lay the foundation for marker-assisted breeding and the early selection of seedlings.