| Total flavonoids were extracted from Limonium aureum (L.) Hill. produced in Gansu, and its extraction process were explored and optimized in the present study. The oral liquid from ethyl acetate fraction of Limonium aureum (L.) Hill. was then prepared and its anti-inflammatory effects and toxicity were evaluated. The main results were as follows:(1)A method used for determinatiing the total flavonoids content by UV spectrophotometry was established. The uniform design was used to optimize the experimental conditions and the related factors effecting on the experiment were analyzed by DPS software. It was found that the main factors effecting the extract rate of flavonoids from Limonium aureum (L.) Hill. were in the order as: ethanol concentration>extraction temperature>liquid ratio>ultrasonic processing times> extraction time. Limonium aureum (L.) Hill. The optimal condition obtained were in the following way: ethanol concentration was 78%, solid-to-liquid ratio 1:32, extraction time 30 min and extraction temperature 80℃.(2) The anti-inflammatory effect of the alcohol extract from Limonium aureum (L.) Hill. was determined by use of the method of xylene-induced ear swelling. It was found that an extremely significant different existed in the weight and thickness of mice ear film between the high-dose group and control group (P<0.01), a significant different between low-dose group and the control group (P<0.05), while no significant between the low-dose group and positive control group (P>0.05). The results indicated that two doses of ethanol extract from Limonium aureum (L.) Hill. could inhibit the ear swelling in mice.(3) The anti-inflammatory effect of the oral liquid of ethyl acetate from Limonium aureum (L.) Hill. were tested by mice granuloma induced by cotton embedding method . It was found that a significant difference existed in granuloma weight between high-dose group and control group (P<0.05), almost the same as aspirin Lin group (P>0.05), while no significant between low-dose group and control group (P> 0.05). It indicated that the oral liquid of ethyl acetate from Limonium aureum (L.) Hill. could inhibit the granuloma in mice.(4) Toxicity tests showed that no poisoning symptoms were found 1 week late in the mice given a maximum dose (8 g/kg) of the methyl alcohol extract from Limonium aureum (L.) Hill. The maximum tolerance dose (MTD) was 15 g/kg> 10 g/kg. Chronic toxicity test showed that the extract of Limonium aureum (L.) Hill. had inhibitory effect on the body weight of wistar mouse, no significant effect on the physiological factors of blood; while had pathological damage to major organs which became stronger with increasing concentration of the extract. It indicated that Limonium aureum (L.) Hill. had certain chronic toxicity.
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