Study On Bacteriostasic Activity & Anti-oxidation And Optimum Extraction Of Total Flavonoids In Jasmine Flower Residue

Jasmine is a sort of senior spiceberry.Jasmine flower residue is flower which lost fragrance by fumigating scented tea and it was made to waste materials.The tea only absorb a part of fragrance of jasmine flower on fumigating.There are containing a great deal valuable sweet-scanted materials and micreelement and are great value in use.Modern industry of scented tea brings ahout a large amount of flower dregs.Till now some experiments have been conducted to promote its comprehensive utilization,the result is not ideal.Jasminum flavonoids was-mainly studied in the experiment.Extractive optimum condition of Total flavonoids in Jasmine flower residue were probed into.Anti-oxidation and Bacteriostasic activity of Total flavonoids in Jasmine flower residue were reviewed.The conclusion was educed by a series of experiment.The content of total flavonoids in Jasmine flower residue was mensurated by circumfluence.The standard curve was made.there was a good linear relation shipwith R²= 0.9996 between the absorbance and the concentration of rutin.It was showed that the content of total flavonoids in Jasmine flower residue was 54.45 mg/g.By means of extracting using organic impregnant,the extravtive that contained 21.5%flavonoid was got,and it contained flavone and flavonol by identifying using color reaction.The experimentation showed that total flavonoids extractive had the ability to clear O₂^-·and the ability was obviously increased when concentration of total flavonoids extractive was increased.Total flavonoids extractive showed distinct anti-oxidative activity in lard emulsion.The anti-oxidative activity of 0.1%extractive was stronger than 0.1% vitamin C.The anti-oxidative activity of 0.3%extractive was stronger than The anti-oxidative activity of 0.1%and 0.2%.So the anti-oxidative ability in lard emulsion was obviously increased when concentration of total flavonoids extractive was increased.Bacteriostasic experiment of total flavonoids was showed that the flavonoids have obvious bacteriostatic bioactivities against Staphyloccocus aureus Rosenbach and Escherichia coli.ti was shouwed that Total flavonoids in jasmine flower residue had obviously Bacteriostatic activity.The result showed that 70%alcohol solution was more propitious for extractiving of flavonoids by extractive experiment hot water with 70%alcohol solution.By means of orthogonally rotational combination design,a four-factor(temperature,time,concentration in alcohol,ratio between materials and water)substrate cultivation experiment was conducted by using Jasmine flower residue.The regression equations of the relationship between temperature,time,concentration in alcohol, ratio between materials and water ang extraction rate of total flavonoids in Jasmine flower residue was established.The results indicated that extraction rate of total flavonoids in Jasmine flower residue was affected obviously in successive order by temperature, concentration in alcohol,ratio between materials and water and time.In range of experiment,extraction rate of total flavonoids was increased obviously when temperature and time were in higher levels,when temperature and concentration in alcohol were in higher levels and when concentration in alcohol and ratio between materials and water were in higher levels.And the interaction between temperature and time,between temperature and concentration in alcohol and between concentration in alcohol and ratio between materials and water.Considering the high extraction rate and activity of total flavonoids,the extraction conditions were optimized by frequency analysis that were 77.84～79.04℃temperature,86.22～97.40min time,79.22～83.43%concentration in alcohol and 1:31.51～1:34.40 ratio between materials and water.