Studies On Tissue Culture Shoot Regeneration In Vitro Of Taishanhong Punica Granatum L.

The paper summarized research progress on tissue culture of fruit tree. Crucial technology of multiplication, seedling strengthening, rooting, organic compounds on tissue culture, leaf in vitro culture of Taishanhong Punica granatum are studied in detail.The further studies,such as genetic transformation and polyploidy breeding of Punica granatum would based on this paper.The results indicated:1. The most suitable multiplication medium was optimum with MS + 6-BA 1.2 mg·L^-1 +IBA 0.1 mg·L^-1,the multiplication index of shoot was 3.1. If the concentration of cytokinins continues to enlarge, after sub-culture, the young sprout become weak, the stanza is thin long, leaf area lets up etc. 'glass'phenomenon, disadvantage in multiplication of explants.2. The best rooting mediums were optimum with half-strength MS+NAA 1.2 mg·L^-1,the growing rate of root was at 73%. The NAA and IBA could induce different appearance roots, The root that NAA induce grow better and robust, but the hair root that IBA induce and thin long.3. The strong seedling result of MS₀ surpasses the 1/2 MS, but multiplication index is low. Cytokinins 6-BA uses alone, strong seedling result is worse;When 6-BA and NAA to match with the usage, strong seedling result is better;The PP₃₃₃ could certainly promote the strong seedling.the optimum culture medium for strong seedling is MS + 6-BA 1.0 mg·L^-1 + NAA 0.1 mg·L^-1 and this medium is also used as sub-culture medium. In addition, the apical meristem (1cm or so) that chooses the opposite strong seedling carries on the strong seedling culture, the result is very good.4. Coconut juice had the most significant effect on the survival ratio of the explant.200 mL·L^-1 of coconut juice could prohibit topmost blasting triumphantly.5. The result showed that cytokinins is necessary for Tai shanhong Punica granatum leaves to regenerate in vitro shoot and the optimium medium was MS + 6-BA 4.0 mg·L^-1 + IBA 0.3 mg·L^-1 + GA₃ 0.3 mg·L^-1 and MS + KT 1.0 mg·L^-1 + 6-BA 2.0 mg·L^-1 + GA₃ 0.5 mg·L^-1, the shoot reduction frequencies reached up to 52...