Study On The Histology And Immunohistochemistry Of Laiwu Black Goat's Pineal Gland And Hypothalamus

Laiwu Black Goat is the superordinary endemic breed of Shandong province. It has extensive purpose such as meat,velvet,feather and leather. Its main production function surpasses other goat specieses, the meat quality is better, the amount of velvet is higher, furthermore,it is accommodate the grazing condition of mountain area.The sexual maturation of Laiwu Black Goat is approximately 4-5 mouth. Its initial mating is approximately 10 mouth. the oestrus cycle is approximately 15-20 days. In order to raise reproductive ability,we should know the change of pineal gland,and the relation of structure and reproductive.GnRH neuron in hypothalamus can secrete GnRH,which is very important in genito- endocrine. Apply SABC method to research to the distribution of the gonadotroph cell and positive material in the ovary, provide the morphology materials for reproduction and physiology.TEST 1 Immunohistochemical Comparison of the Laiwu Black Goats Pineal Body Functional Status in four seasons Laiwu Black Goat's pineal bodies from four seasons are investigated by immunohistochemical SABC method.The results are as follows:⑴A great number of 5-HT-containing cells and fibers are observed in the pineal body.⑵when the four seasons are compared:the area of pinealocyte and rate of 5-HT-containing area in winter are obviously higher than other seasons (P<0.01)).Summer is lower than other seasons.Spring and autumn are almost the same(P>0.05) .The OD value of the four seasons was almost the same(P>0.05). As a result, the functional status of Laiwu Black Goat's pineal body changes with the season ,which has intimate relation with goat breeding activity.TEST 2 Comparison of the Laiwu Black Goats Pineal Body ultrastructure in four seasons Observe Laiwu Black Goats pineal body ultrastructure by TEM, The...