Cloning And Sequences Analysis Of PRL Gene From Muscovy Duck

Broodiness is one of the primary factors to influence the reproduction ability of the poultry, and Prolactin is the key hormone to the broodiness of the poultry. It is important to do some reseach on the mechanisms of regulation of the PRL gene from levels of molecular. The total RNA of anterior pituitary glang was extracted from Muscovy duck, and the reverse-transcribed product was subjected of PCR amplification, in which the primers were designed based on the sequence of Pekin duck(Anas platyrhynchos) prolactin gene cDNA. The specific fragment was cloned and analyed. Accordingly,this study provides some informances for knocking out the genes of broodiness,which can quicken the genetic process and increase the feeding benefits.(1)The complementary DNA(cDNA) of prolactin (PRL) was cloned and sequenced (GenBank accession number: AM180924). The complete nucleotide sequence is 765bp,encoding 229 amino acids, whose molecular weight is 25.8 kDa, which can help study the polymorphism ,expression and regulation of PRL gene , the relations between broodiness and PRL gene can aslo be investigated.(2) By comparing with published sequence in Genebank with BLAST, the sequence showed hightest identity with PRL gene. It is predicted by Signal IP that of the complete amino acid sequence,the first 30-31aa is signal peptide. which had highly homology identities among different species.(3)Homology analysis suggested that the homology of Muscovy duck PRL coding region with Pekin duck PRL coding region was 99%, and was found to have 98. 3%, 98. 1%, 98. 3%, 91. 3%, 92. 4%,and 91. 0% with Magang goose,Rhine goose, Wanxi white goose, Chicken, Turkey, and Quail, respectively. The predicted amino acid seqence had an overall similarity with a comparable region of Pekin duck (98. 7%), Magang goose (99. 1%), Rhine goose (98. 3%), Wanxi white goose (98. 7%), Chicken(91.7%),Turkey(93.3%), and Quail(93.0%) PRL.The study showed Muscovy duck is much more closer related to Pekin duck than goose , chicken, turkey and quail, which indicted the PRL gene is conserved accordence to the evolution...