Muscovy Duck Broodiness Behavior Of Differentially Expressed Genes And Trait Association Analysis

To duck as the experimental samples, using cDNA-AFLP technology using 64 pairs of cDNA-AFLP primers duck on the nest to nest and non-detect polymorphism between the screening differentially expressed genes. And using candidate gene approach to GnRH, GH and FSH genes duck egg production performance of the three molecular markers.1. Using cDNA-AFLP method was used to duck in on the nest to nest and non-differentially expressed genes, there were bands, of which about 82 differentially expressed fragments from cloned 15; through the database than on the analysis, including eight genes with known or presumed obvious homology 2 genes of unknown function homologous to 5 and the database of existing genes no significant similarities. 3 were randomly selected from RT-PCR validation, the results were identical with the cDNA-AFLP2. In the eight genes with known or presumed existence of significant homology of the fragments was confirmed in poultry egg production performance-related genes, including GnRH, GH and FSH 3. There are some other genes, such as superoxide dismutase, keratin related protein, thiocyanate enzyme release S-transferase enzymes.3. Muscovy candidate gene detected two pairs of primers of polymorphic loci are both located in Hardy-Weinberg equilibrium.4. SSCP method of analysis using GnRH gene polymorphism associated with the performance of muscovy duck egg, duck proved GnRH gene polymorphism was significantly related to egg production.5. SSCP method using GH gene polymorphism associated with muscovy duck egg production analysis showed that GH gene polymorphism and not between egg production was not significantly related.6. SSCP detected using two gene polymorphism and duck production up even the correlation between the number of days and found that even the longest production days of duck and polymorphism between the two genotypes were significantly associated.