Study On The Improvement Of Rice Straw Ruminal Degradation With Agaricus Blazei And Valvariella Volvac Pre-culture And Its Mechanism

In this work, three experiments were conducted to evaluate the effect of Agaricus blazei and Valvariella volvac pre-culture for rice straw on improving straw ruminal degradation and impacting oi Agaricus blazei and Valvariella volvac to rumen microbial ecology; on the bases of evaluation, the mechanization of A.blazei and V.volvac pre-culture was discussed on upgrading straw degradation.In experiment one, the effects oi A.blazei and V.volvac treatments on improving degradation quality were compared with in sacco technique for different pre-culture duration. Results showed that, compared to V.volvac treated and untreated groups, the DM degradability oi A.blazei treated straw was increased significantly for about 15.17% (P<0.05) and 15.42% (P<0.05) respectively. The longer the A.blazei was cultured, the higher the degradability was obtained. The content of Hcel and Cel in residue were lower in A.blazei treated group than in V.volvac group for 8.03% (P<0.05) and 7.83% (P<0.05), respectively. The kinetic degradation revealed that the soluble (a) and degradable (b) proportion of A.blazei treated straw were increased about 12% and 15.5% respectively, and its degradation rate (c) had improved for about 0.0152. On the contrary, there was no significant improvement on degradation was observed for V. volvac treated straw (P>0.05).In experiment two, the kinetic tendency of in vitro degradation was analyzed for A.blazei and V.volvac treated straw in aspects of pH, VFA, NH3-N and MCP. Comparing with V.volvac, Tp and ck groups, a remarkable effect on improving DMD was obtained by A.blazei treatment. Based on ck group, the DMD of TA3, TA2 and TA1 was improved for 78.25% (P<0.05), 58.74% (P<0.05) and 24.68% (P<0.05), respectively. V.volvac treatment, however, was not significantly different from Tp and ck (P>0.05). Comparing of kinetic tendencies, the peak of pH was reached after 8h incubation, except Tp group that was in 4h; the fluctuation of pH was less in A.blazei group than in V.volvac. The kinetic tendency of NH3-N was similar revealed by different treatments. Moreover, the propionic acid content of in vitro incubation was higher in A.blazei group...