Identification Of Mycoplasma Capri Isolated From Caprine Mycoplasmal Pneumonia

Two kind of pleomorphism microorganisms (named Y₁ and Y₂) were isolated from the lung tissues and trachea of infected cattle from a farm in SiChuan. They were indentified as Mycoplasma capri by the following methods, culture characterization, morphological tests, biochemical reactions, growth inhibition tests, metabolism inhibition tests, serological identification and animal experiments in goats.Results of above experiments indicated that both microorganisms could be lautered by micr-opore filter of 0.45μrn aperture. And the lautered products could be serially subculti-vated. In morphological tests, both microorganisms were hard to be dye by Gelan Dye. In contrast, they showed amethyst by M-Wright's stain. Under the eyesight of transmission electron microscope, they both showed polymorphism, such as globe, thread, pole, spire and so on. The size of them was between 0.2-0.4μm. During the course of cultivation, conglobate and petite colonies like dew point on solid medium with 1% nutrient agar could be obwerved. If observed with 40 times magnification, though they had tiny difference, the shape of them were all like fried egg. The colonies of Y₁ with compact protuberance in the center of which were small and compressive to mix together. In contrast, the colonies of Y₂ were bigger, dispressive and with no gibbous center hylum.The results of biochemical tests indicated that they were positive with Glucose fermentation( + ), Equine serum liquifaction ( + ), Methylene Blue disoxidation ( + ), and negative with Urea hydrolysis (-) ,Arginine hydrolysis (-) , Film and pot form (-) , PhosphataseActivity (-) . The indirect hemagglutination tests showed that the antigen of Y₁ and Y₂ could agglutinate with positive serum of PG₃ and Y₉₈; not agglutinate with negative serum of PG₃ and Y₉₈.G rowth inhibition tests demonstrated that the positive serum of PG₃ and Y₉₈ could inhibit growth of Y₁ and Y₂ to form inhibiting collar of diameter exceeding 2mm.In the contrary, the negative serum of PG₃ and Y₉₈ could not inhibit the growth of Y₁ and Y₂.In the metabolism...