Isolation And Identification Of The Agents Of Contagious Caprine Pleuropneumonia (CCPP) In Guangxi

Contagious caprine pleuropneumonia (CCPP) is an highly contagious disease ofgoats, caused by Mycoplasma spp., characterized by high fever, coughing, serous andfibrinous pleuropneumonia. In the recent years, suspectable CCPP cases always happenedin Guangxi. But agents couldn't be isolated.In this project, firstly an epidemiological survey was performed on the status of CCPPin Guangxi. The results showed CCPP mainly happens between July to September andaffects young goats of one year old or so, the average incidence and mortality were35.8%(from 26.67% to 56.25%) and 27.5% (from 16.67% to 50%).,and 668 goat serumsamples were tested by Minim Indirect Haemagglutination test (MIHA) for detecting theAntibodies of M. ovipneumoniae and M. mycoides subsp, capri The antibody positiverate of M. ovipneumoniae and M. mycoides subsp, capri were 13.3%(89/668) and 18.7%(125/668) respectively,, and 60(8.98%) samples were both seropositive to M.ovipneumoniae and M. mycoides subsp, capri.4 isolates of Mycoplasma spp. were isolated from 5 samples of diseased goats mainlycharacterised by respiratory signs in Nanning and Liu Zhou. The isolates were M.ovipneumoniae and M. mycoides subsp, capri identified by morphology, culture character,biochemical tests, growth inhibition test, metabolism inhibition test and animalexperiment, .It's first time that the pathogens of CCPP were identified in Guangxi. And atthe same time, it was confirmed that CCPP was caused by M. mycoides subsp, capri, and M.ovipneumoniae together.PCR primers to M. mycoides subsp, capri, and M. ovipneumoniae(which gene?) werecomposed respectively according to the references. Four isolates, 8 strains and 20 sampleswere tested by PCR. Segments in genes of M. mycoides subsp, capri, and M.ovipneumoniae were amplified in all of the 4 isolates. A segment in gene of M. mycoidessubsp, capri, was amplified from strains GX1~#-1,GX2~#-1,GX4~#-1 and GX5~#-1. A segmentin gene of M. ovipneumoniae was amplified from strains GX1~#-2,GX2~#-2,GX4~#-2 andGX5~#-2. Fourteen of 20 samples were positive by PCR test. A segment in gene of M.ovipneumoniae was amplified in 10 samples and a segment in gene of M. mycoides subsp. and M. ovipneumoniae were amplified in 6 samples. CCPP in Guangxi and its agents werediagnosed by molecular biological techniques.Inactive vaccine of isolate from CCPP infected goat was produced and was provenqualified by sterility test, safety test, potency test and field tests. And it got good feedbacks.So it can enters the experimental stage.