Detection Of Oxytetracyline Residues In Food Of Chicken And Duck By HPLC And Study On Accumulative Toxicity

Oxytetracydine was Wide table antibiotic found in 1950, constraining blue positive fungus, negative fungus, spirillum, clothing former body, etc. As antimicrobial, it was extensively used for swine, ox, chicken, duck in domestic and international veterinarian clinically, and as forge additive to add prevent disease too. Because of extensive application for a long time, bacterium engendered the resistance to drugs, and dosage also improved constantly. Because of abuse in long time, residues in beasts and birds tissue increased constantly, leading to great endanger after being eaten by peopie. It already both at home and abroad reported that it can cause "infect dually", bone Qia's teeth turn yellow, foetus' death. It can also cause malformation and cancer for a long time use.Domestic and international residual research to terramycin, the majority stays in the single analytical method," double infection "," tetracycline yellow tooth ",etc. and the toxicity to people and animals and system research of fast detection are rare report testing acc production performance ,clinical symptoms after animal take orally different dosage of Oxytetracycline, cut open and examine changing, histology slices, the biochemical index of blood plasma and measuring Oxytetracydine remains in tissue with the modem most advanced instrument high-efficient liquid phase chromatograph (HPLC ). Then the meat duck take orally medicine to make repeated experiments in one month with age of 2 weeks, explore the toxicity, toxic mechanism, target organ of terramycin, and the absorption, distribution, excreting for further study, in order to offer scientific basis for detectingChicken's accumulating toxic test result: Buy 100 branch hybrid chicken at the age of two weeks from local chicken field that unexposed to any medicine, feed the mixed feed not adding any medicin . Through immunity vaccination, make antibody after feeding two weeks, choose No. 20 Branch to be divided into four groups at random: Group O (control group), test I group (low dosage), group II (China's dosage) and group III (high dosage), every group No. 5 Branch, according to design dosage giving each group take orally medicine12.5mg, 25mg, 50mg, increase dosage with a time every week with weight later. The change after offering medicine: (1) Symptom before death : 4 day I , II,, III group appear poor in spirit successively, the appetite decreasing, addicted to sleeping. Group IIIchicken die No. 2 Branch in the seventh day and the eighth day, Group II die 1 branch, one of death of group I for the 14th day. Other chickens have depressed spirit ,and the appetite is reduced by half or abolished definitely, does not get up lying , eyes half-closed or close all, cockscomb, and face pale, arrange rare to relieve oneself. (2)Cut open and examine changing: Cut open and examine the natural death chicken in one month in time, it is swelling seriously. It is 1.5- times of the normal liver, the blunt garden of edge of liver have greasy senses fragily. The kidney is 1.2- 1.5 times more swelling, bleed and...