Studies On The Regeneration System Establishment Of Mei Flower(Prunus Mume)

With the development of genetic engineering ,it offers potential for rapid crop improvement through the use of techniques involving the direct integration of genes into using vectors in hardy mei flower breeding work. However, the successful regeneration of plantlets from transformed tissues remains a major limiting factor that integration of exogenous DNA into plants would be only of immediate benefit if it occoured in a known and already selected genotype. For this, work with mature explants is clearly very important. Our studies focus on constructing an useful combination of different hormone and environment condition, so that we can afford an useful regeneration system of mei flower.The whole test can be divided into two parts, one is for the culture of some mei flower cultivars in vitro because it can afford the materials for the next step; the other for the regeneration of the mature leaf tissue.In the test, 8 cultivars of mei flower are prepared to culture in vitro. Three of them are‵ tieguhong′,‵ meirenmei′and‵yanxing′.Through a serials test, the growth of explants ,proliferation and the rooting culture of the plantlet achieved.Of all the 8 cultivals,‵ tieguhong′was in troble.We find a modified media: Q&Lmacro elements+Pmicroelements+MS organ+2×MS iron salt+AgNO3 4 mg/L+glucose 30g/L .The result of the test is: the optimum proliferation meida is modified Q&L+6-BA1.0+NAA0.05+GA30.5(mg/L)(the use of AgNO3 should be attached great attention ); the optimum rooting media is modified Q&L+NAA0.3+IBA0.1(mg/L).The media of‵ meirenmei′is: proliferation:WPM+6-BA1.0+NAA0.1(mg/L),rooting:1/2WPM+NAA0.5(mg/L).The media of‵ yanxing′is :Proliferation MS+6-BA0.5+IBA0.05(mg/L),rooting:1/2MS+IBA0.5(mg/L).In the second test,‵meirenmei′is used as the test material. The result is: the procession of the whole test should be divided into two parts, on is for the inducement of the leaf callus, the other for the regeneration of the leaf callus. In the first step, 2,4-D play an important role in the inducement of the callus. The darkness culture condition and the liquid inducement media are better for the growth of the callus.The optimum media of the inducement is 1/2WPM+2,4-D 5mg/L. After 7 days inducement, transfer the leaf explants to the fresh media : 1/2WPM+6-BA1.0 mg/L+NAA0.1mg/L+AgNO38 mg/L+LH 100 mg/L,then the regeneration rate can ahieve 24.25%.Though seldom articles on mei flower tansgene were issued in China, this study provid foundation for the lasting research.