Plant Regeneration Of Peach From Leaflet Cultured In Vitro

In order to optimize tissue culture system of peach (Prunus persica L.) and to improve plantlet regeneration rate and to establish high efficient plant regeneration system from leaves, leaves was used as explants on the basis of establishment of a rapid technique system of stable and high efficient micropropagation. Wild peach seedling [Prunus persica (L.) Batsch. ], Gansutao (P. kansuensis Rend.), Sunagowase [P. persica (L.) Batsch. ], Shuguang (P. persica var. nectarina Maxim) and Zaolupantao( P. persica var. platycarpa Bailey) were used as tested materials. The results were as follows:(1) A rapid technique system of micropropagation In Vitro culture was established. The effect of sampling time, basic media, cultural condition and the method of disinfection on pre-culture were distinguished. The results were as follows: As to Shuguang, Sunagowase, Wild peach sideling, and Zaolupantao, explants were sampled on March, April, May, September and October, the rate of germination was very high, but to Gansutao, sampled from March to October, the rate of germination kept higher than 79% and no significant difference. Ideal pre-culture media was LP media, the rate of germination was 210% of MS media; light intensity of 10001x was beneficial to explants germination; the project of disinfection in which the rate of contamination was 2% and injury very little was proved up.The research was focused on basic proliferation media and 3 types of NAA and BAP combination or TDZ and NAA combination and pH in continuous 3 times subculture. In conclusion, the optimum media was LP media with 0.75mg/L BAP and 0.3mg/L IBA for Shuguang, Sunagowase, Wild peach sideling and Zaolupantao, but SH with 0.75mg/L BAP and 0.3mg/L IBA for Gansutao. The favorable pH for proliferation culture was 5.4-5.8 for peach.(2) A high efficient regeneration system from leaves was established. The technique and condition of adventitious shoot from leaves in Vitro culture were studied. The effects of 3 kinds of different source of leaves, regeneration media, leaves maturity and material on the position of crown of a tree on regeneration were analyzed. The results showed that, with ideal explants from the tender leaves of adventitious shoot the regeneration rate was the highest-45%. But from proliferated leaves or pre-conditioned leaves, the regeneration rate was under 33%. The better regeneration media was SH+TDZ3.0mg/L+KT0.3mg/L. The regeneration rate of 1.0-1.5cm long fully expanded leaves was 3 times than 0.5-1.0cm long. The material from the bottom of crown of a tree was propitious to adventitious shoot regeneration from leaves. All the plantlets developed into complete plants with normal morphological characteristics.(3) The adventitious shoots were rooted and plantlets were obtained. The effects of cultural condition, basic media and hormone on the rooting were studied. The results were as follows: After 10 days of 3001x light intensity treatment transferred to 25001x light intensity and cultured for 20d, amount of effective roots were 3.86 per shoot and the rate of rooting was 100%.The best rooting medium was 1/2LP supplemented with 1.0mg/L NAA and 1.0mg/LIBA.