Study On Male Sterility Of Tagetes Erecta L. By RAPD Molecular Marker

Marigold is the popular flower used in the modern landscaping and also the valuable economic plant. The research developed a quick method to extract DNA from marigold male sterile line, fertile line, maintainer line and F1. And a RAPD reaction system suitable for marigold was developed by thrice orthogonal experiment designs. Secondly, the results showed that the primer OPA18 can be considered as the marker to identify the seed purity of marigold stably.The identification data of seeds purity by RAPD technique using primer OPA18 almost accorded with the regular identification data of it in the field. Thirdly, using RAPD technique to amplify DNA of the AALY001 NMS two lines, one specific marker NMSOPM09500 was obtained in the male fertile line but without in the male sterile line. This marker was stable after repeating amplification. Therefore, it is helpful to identify seed purity and it also provides technical support for the research on male sterile gene of male sterile marigold by RAPD marker.