Study Of The Fertility Change Of The Uplandcotton After⁶⁰Co Irradiation

Cotton occupy an important position in the agricultural economy, improve cotton production is the most significant way to heterosis utilization. Looking for screening suitable cotton male sterile line, supporting the implementation of three lines, is the current cotton heterosis research an important way. Radiation treatment is to produce male sterile mutation of the most effective means of mutation, with small changes in the genetic background, the test cycle is short, and other advantages. The purpose of this study is the use of60Co γ-ray radiation screening upland male sterile mutant male sterile line breeding as to provide a basis.In this study, our group is the existing20kinds of upland cotton material, the use of60Co γ-ray irradiation, through artificial selection, microscopic examination, physiological and biochemical parameters were measured and used to correct the difference between the DNA level approach fertile plants screened suitable sterile mutants to compare their traits, compared to the implicit rules of the indicators. Of the following conclusions:1, the use of different doses of60Co γ-ray irradiation treatment, all varieties of pollen sterility are improved. Each material have both been completely sterile mutants. Ml generation,0.442kGy irradiation of09-32infertility highest rate of33.33%. M3-generation groups and non-fertile plants close to a3:1ratio of sterile plants, initially determined to one recessive genie male sterility gene ms control mutants.2, physiological and biochemical indicators show:Sterile Mutant leaves soluble protein than wild-type and M2generation, soluble sugar content than the wild-type and M2generations. The COX, SOD, CAT and POD enzyme activity such as in no significant differences between the three materials, the more obvious differences between the anther.3, using random amplified polymorphic DNA marker technology, research and wild-type,6Co γ-ray radiation mutagenesis male sterile mutant, M2DNA level differences between generations. In the20primers screened out of the six clearer primers amplified38polymorphic loci great help to improve breeding efficiency.