| The optimal conditions for the extraction of total flavonoids from Indocalamus leaves with methonds of water-bath and ultra sonic wave were investigated in this study. Meanwhile, the contents of five kinds of chemical compositions, the total flavonoids, volatile composition, glucide, protein and metallic elements were measured in the leaves from the same place in two different seasons (summer and winter) or from two different places in the same season (summer) or from the same place in the same season (summer). Thereby, the influence of the season, soil environment, botanic growing conditions and breeds ( Indocalamus latifolius and Indocalamus tessellaues) on the contents of each composition were determined.The optimal conditions for the extraction of total flavonoids from Indocalamus leaves with water-bath method were:ethanol concentration 95%, extraction for 3 h at 85℃ . The % yield of the extract went up as the increase of the ratio of solvent and material. So for saving the cost, 25:1 of the ratio mentioned above and 85% of aqueous-ethanol were used in the work. The optimal conditions for the extraction of total flavonoids from Indocalamus leaves with ultra sonic method were: 85% of aqueous-ethanol, 20:1 of the ratio mentioned above, and the % yield of the extract went up as the increase of the extracting time, so the extracting time was 30min in this work. In addition, the % yield of the extract went up as the increase of the extracting times. The % yield of the total flavonoids extraction made up much the same with the two methods, but the efficiency of ultra sonic method was higher.The total flavonoids contents of Indocalamus leaves was1.38%-1.92%. All the factors of the season, soil environment, botanic growing conditions and breeds had influence on it. It was higher in winter than that in summer, the total flavonoids contents of Indocalamus tessellates was higher than that of Indocalamus latifolius and the soil environment and botanic growing conditions only made small difference.
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