Effects Of Growth Regulating Substance On Relative Enzyme Activity In Nitrogen And Sugar Metabolism And Yield And Quality Of Sugar Beet

By using Diplontic clonal sugar beet variety Tianyan 7 and main plant varieties Shuangfeng 319 and combining the methods of pot planting and field trial, the effect of growth regulating substances S-ABA (S-absic), G-ABA (gibberellin and absic) and DA-6 (hexanoic acid 2-(diethylamino) ethyl ester) used under different concentrations and in different spraying period on the activity of Nitrogen and Sugar metabolism-relative enzyme(nitrate reductase (NR), glutamine synthetase (GS) and saccharase (SS)) and their associated product were researched and the yield and quality of sugar beet ,the suitable kind, dose and best spraying stage of growth regulating substance were screened out. The physiological basis of the effects of growth regulating substance on nitrogen and sugar metabolism of sugar beet were explored in order to achieve effective accumulation of sugar on condition of the lowest carbon consuming and to provide the theorical reference for taking effective regulating measures in sugar beet production. The results were as follow:When the concentrations of S-ABA was in range of 10 to 35mg/kg, NR activity (NRA) and GS activity (GSA) of leaves and root gradually enhanced with the concentrations of S-ABA increasing, GSA reduced while the concentrations was more than 35mg/kg. 35mg/kg was most suitable for SS synthesis activity and 30mg/kg was the best for SS decomposition activity. The effective concentrations of increasing sugar yield was 30mg/kg toTianyan 7, and Shuangfeng 319 was 35mg/kg, the same time, ash (K+ , Na+) andα-amino nitrogen content in dray matter descented.The yield and quality of sugar beet could be significant improved while the concentrations of G-ABA between 20mg/kg and 25mg/kg, the effective concentrations of Tianyan 7 was 20mg/kg, and of Shuangfeng 319 was 25mg/kg. Different concentrations adapted to different enzyme activity, the SS decomposition activity was enhanced in root, NRA and GSA in functional leaves under concentrations of G-ABA was 20mg/kg, and SS synthetic activity in root was enhanced under 25mg/kg..While the concentrations of DA-6 was between 7.5mg/kg and 10mg/kg, yield and quality was increased, the favorritest concentrations of Tianyan 7 was 10mg/kg and Shuangfeng 319 was 7.5mg/kg. Each concentrations of DA-6 could significant reduced the ash (K~+ , Na~+)andα-amino nitrogen content of shuangfeng 319. The effective concentrations on NRA and GSA was 10mg/kg. The effective concentrations on SS synthesis activity was 7.5mg/kg, and on decomposition activity...