| The regeneration systems of Emerald Seedless and Thompson Seedless were established by organogenesis and somatic embryogenesis, respectively. The novel stilbene synthase gene which was carried in the pWR306 binary vector was introduced into leaves and petioles of Emerald Seedless, into petioles of Long Thompson Seedless, into somatic embryos and embryogenic callus by Agrobacterium-mediated. Concentration of hygromycin for selection, concentration of cefotaxime for wiping off bacterium, concentration of Agrobacterium and time of pre-culture were studied. The foreign genes were confirmed by selection of hygromycin, PCR analysis and Southern blotting. The mainly results were:1 Producing of regeneration system(1)Organogenesis system Regeneration system from leaf and petiole of Emerald seedless grape using thidiazuron was studied. The results were: Regeneration from leaf was easier than from petiole. The combination 2.5mg·L-1 TDZ with 0.1mg·L-1 NAA resulted in the highest frequency of adventitious bud production from leaf. The adventitious bud regenerating rate is 21.57%. The medium MS+TDZ 1.0mg·L-1 was the best for bud regeneration from petiole with regeneration rate of 2.08%. The optimum medium for bud elongation is MS+TDZ 0.25mg·L-1 + NAA 0.01mg·L-1.The medium 1/2MS+IBA 0.1mg·L-1 was better for roots inducing.(2)Somatic embryogenesis system Somatic embryogenesis and plant regeneration from anthers and ovaries of Thompson Seedless (Vitis vinifera L) were studied. The results were: The best combination of hormones for embryogenic callus inducement of Thompson Seedless from anther and ovary are BAP 4.0 mg·L-1,2,4-D 0.5 mg·L-1 and BAP 4.0mg·L-1,2,4-D 1.0mg·L-1, respectively. Somatic embryos are induced on embryonic callus inducing medium but no growth regulator. The plantlets and new embryoids were produced on free-hormone MS medium. The medium WP+sugar 15g·L-1 was the best for complete plant forming.2 Improvement of genetic transformation system...
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