Inhibit Procambarus Clarkii From The Infection Of WSSV By WSSV Reconstructed Viral Attachment Protein 1 (rVAP1)

White spot syndrome virus (WSSV) is one of the major pathogen of global shrimp farming. WSSV has currently been a major threat not only to shrimp culture but also to marine ecology. Since 1992, a lot of studies have been performed about WSSV and WSS. The pathogenic moleucular mechanism is not known, and viral infection in shrimp has no effective therapeutic or prohylactic counter measures.A cellulose nitrate membrane was laid on top of the plate in order to quickly screen high-expressing clone. High-expressing reconstructed WSSV rVAP1 (Viral Attachment Protein one) clone was selected and expressed successfully by yeast expressing system. rVAP1 expressed by the screened clone was identified by SDS-PAGE and the binding capability of rVAP1 to shrimp membrane proteins. Clone A-27 selected as the high expressing clone was small scale fermented in the labrary. The produce expressed was identified by SDS-PAGE. As the animal model, Procambarus clarkii were infected with WSSV to study the viral titer (median lethal dose). The inhibiting effect of rVAP1 was studied throngh infecting crawfishes with mixures of different dilutions of rVAP1 and WSSV. The results of infection trial shown that: rVAP1 which was infected 1.6μg per crawfish could inhibit the infectivity of WSSV which was diluted under the viral titer of 6.81 LD50 and delay the death time of crawfish.This paper lays a foudation for deeper explanation of pathogenic mechanism. It provids a new approach of exploitting antiviral drugs and feeds, furthermore, offers a useful reference to the disease control and the interrelated research.