Study On The Diagnosic Methods And Integrate Control Of Mousepox

The mousepox in a lab animal center was diagnosed by electron microscope, immunology and molecular biology technologies. This will serve as a theoretical basis and refenrence for the diagnosis and integrated control of mousepox in mice.The investigation of epidemiology shows that the mousepox was caused by transport and the change of climate. The yong , the breeding boom and the male mice are infected more easily than the old and the female mice. The symptoms of mousepox are atypical, the ulcer in mice heads , the four limbs and tails are rarely observed, but the pathological changes including hemorrhage, swelling and necrosis, etc, in liver, spleen, duodenum and small intestine are typical.The experiment of infecting animals: Take the liver, spleen, lung and kindy from the dying mice and grind into cell suspension respectively, then inoculate the BALB/cA mice and KM mice, Wister rats and rabbits by peritoneal injection with the cell suspension. After five days of inoculation, all the infected mice are dead. After two weeks, the rats and rabbits are normal. The results show that the BALB/cA and KM mice can be infected and killed by mousepox virus and the pathological changes in BALB/cA are more typical than that in KM mice. Wister rats and rabbits aren't infected by mousepox virus.The liver, lung, kindy, spleen and intestine of dead mice were fixed with glutaraldehyde and sliced up, a plenty of oval shape viral particles with dumbbell shape core in the cytoplasm were observed with transmission electron microscope.Put the liver and spleen into muller and grind repeatly , then separate and purify them by centrifuger, a mount of oval or ellipse shape viral particles whose size is 160-190×250-300nm were observed with negative-stain electron microscope .The liver, spleen, kidney, lung, pancreas, intestine of mice infected spontaneously and artificially were dected by HE stain, the main pathological changes include: ecchymoma, focal necrosis and increase of multinuclear giant cell in red pulp in spleen; focal necrosis, infiltration of inflammatory cells in portal area and multinucleate giant cell in liver; hemorrhage and necrosis enteritis in intestine; node of microglial in brain; focal ecchymoma, hemorrhage and cytoclasis in heart, lung, kidneyand pancrease.The positive rate of antibody detected by ELISA in 20 mice 47 days after infecting is 100%.The nucleic acid of virus in various tissues of infected mice was extracted and the A-type inclusion body protein of poxviridae virus gene was used as target gene to compose a pair of primers. The nucleic acid was analized with PCR and the result showed...