Effects Of Dietary Nucleotides On The Inflammatory Response And Gene Expression Of Mice

In this study, the effects of dietary nucleotides on the inflammatory response and inflammatory response related changes of anti-oxidation system and nitric oxide were studied; in addition, the possible mechanism was analyzed by using genechip.KM mice (male, 16±2 days) were respectively fed on basic diets supplemented with 0, 0.25% nucleotides for 15 day. Mice were lavaged with physiological saline (control) and CpG DNA or LPS (experimental groups), and were killed 4 or 18h later. The results showed inflammatory cytokine interleukin-1 (IL-1), interferon-γ(IFN-γ) and serum alanine aminotransferase (ALT), intestinal myeloperoxidase (MPO) activities were relatively decreased, and anti-inflammatory cytokine interleukin-10 (IL-10) was increased with nucleotides supplement uncer CpG DNA or LPS stimulation. These revealed dietary nucleotides can help to maintain inflammation anti-inflammation balance, protect mice from inflammatory injury under CpG DNA or LPS immunostimulation.Results also showed the level of glutathione(GSH) and the activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),Na+K+-ATPase were relatively increased and diaminodiphenyl methane(MDA) was relatively decreased with nucleotides supplement. These revealed dietary nucleotides can help to increase anti-oxidation, protect mice from oxidative injury. In addition, the level of nitric oxide (NO) and the activities of induced nitric oxide synthase (iNOS), total nitric oxide synthase (T-NOS) of intestinal and peritoneal macrophage were relatively decreased and constructed nitric oxide synthase (cNOS) was increased with nucleotides supplement under CpG DNA or LPS stimulation (P>0.05).These results were more significant in 4h than 18h, the injury was bigger and the role of nucleotides was more obvious under LPS stimulation.Balb/C mice (male, 11~13g) were fed 0, 0.25% nucleotides-supplemented diet respectively for 15 day. Mice were lavaged with physiological saline (control) or CpG DNA (experimental groups), and were killed 4h later. The Affymetrix genechip results showed the gene concerned material and energy metabolism, antioxidase, inflammatory signal transduction, inflammatory mediator and apoptosis were up-regulated under CpG DNA stimulation. But the gene concerned antioxidase, inflammatory signal transduction, inflammatory mediator, apoptosis were down-regulated and the gene concerned anti-inflammatory mediator, DNA repair were up-regulated with nucleotides supplemented. These results revealed dietary nucleotides, possibly by affecting the material and energy metabolism, and changing the level of free radical, affect inflammatory signal transduction, and further affect the balance of inflammatory anti-inflammatory response, protect mice from inflammatory injury.