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Analysis On Characters Of Tolerance Low-temperature Sweetening And Qualities In Transgenic Potato (Solanum Tuberosum L.) With Antisense AcInv Gene

Posted on:2007-09-23Degree:MasterType:Thesis
Country:ChinaCandidate:J ChengFull Text:PDF
GTID:2143360185962920Subject:Crop Genetics and Breeding
Abstract/Summary:PDF Full Text Request
Reducing sugar accumulated in potato(Solanum tuberosum L.) tubers will increased during storage in low temperature , and produce dark color of chips known as"Millard"reaction which react with the free animo acid of oil when fry in high temperature. It seriously affects appearance quality and edible quality of the potato products. Therefore, it is very important to cultivate new lines which tolerance low-temperature sweetening.In this paper, transgenic lines of potato tubers of Atlantic, Cardinal, Gannongshu No.1, Gannongshu No.2 and Favorita were used as materials. The objective of this study was to analyze the characters of tolerance low-temperature sweetening and qualities in potato tuber. The major results are as following:In the biology character, the transgenic line"Anti-AcInv Atlantic"and"Anti-AcInv Gannongshu No.2"grew stronger, the shape of tuber and leaf were some different from the control.Compared with the control, the potato number per plant of transgenic line"Anti-AcInv Atlantic","Anti-AcInv Cardinal"and"Anti-AcInv Gannongshu No.2"decreased, and yield per plant of"Anti-AcInv Gannongshu No.2"decreased, the yield per acre(kg/666.7m2) and commodity potato rate of all transgenic lines increased.The dry material content of transgenic tubers remained constantly or varied slightly, the total starch and the amylopectin decreased gradually, and the proportions of amylase and amylopectin were lower in the transgenic lines than the non-transgenic lines (CK).The reducing sugar content increased but the extents of increasing of lines were inferior to the control. During storage at low temperature, the mean of the reducing sugar content of"Anti-AcInv Atlantic","Anti-AcInv Cardinal","Anti-AcInv Gannongshu No.1","Anti-AcInv Gannongshu No.2"and"Anti-AcInv Favorita"were lower 12.20%, 4.88%, 14.63%, 8.11% and 12.12% than the non-transgenic lines(CK). Stored at 4℃for 105d, the reducing sugar content of"Anti-AcInv...
Keywords/Search Tags:potato (Solanum tuberosum L.), AcInv antisense gene, low-temperature sweetening
PDF Full Text Request
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