Study On Exploring Novel Genes For Resistance To Stripe Rust And Good Quality Determining HMW-GS In Synthetic Wheats

With the development of wheat breeding, many studies indicated that narrow genetic basis of wheat resources not only had become a limiting factor to the improvements of the wheat yields and quality,but also enhanced the biotic and abiotic frangibility. With mass of useful genes and rich genetic variations, wild relative of wheat has played an important role in the process of improving the genetic basis of common wheat. So the exploitation and utilization to the key genes in wild relative of wheat is significant to broaden the genetic basis of wheat breeding and sustainable wheat production.The objectives of the present study were to explore novel genes for resistance to stripe rust and good quality determining high molecular weight glutenin subunits (HMW-GS) in synthetic wheats which were kindly provided by the International Maize and Wheat Improvement Center (CIMMYT).The results indicated that:1. The compositions of HMW-GS in 106 synthetic wheats were analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method.Large ranges of allelic variations at three complex loci (Glu-A1, Glu-B1 and Glu-D1) were found. There were three allelic variations among which "Null" allele and subunit 2* were the most frequent at Glu-A1 locus. Five allelic variations were found at Glu-B1 locus, and the subunit combinations 7+8 and 14+15 , which were very important to the bread-making quality ,were the most frequent among these allelic variations.Glu-D1 locus showed the largest range of allelic variation and 19 allelic variations were found.Significantly, Glu-D1 locus might code an unknown novel subunit, which had the molecular weight less than that of subunit 12 which has the least molecular weight so far found in common wheat, and Glu-D1 locus might code one (1Dy) or three subunits .Compared with common wheat, the heterorygosity observed in some accessions at the allelic loci indicated that synthetic wheats had the potential genetic differentiations.2. 102 synthetic wheats were tested for their resistance to wheat stripe rust by inoculating...