Research On The Rooting Shoot Of Test-Tube Seeding And Somatic Embryogenesis From Leaf Balde Of Long Handsome Chinese Flowering Quinc

Chinese flowering quince includes rich nutrients, therefore our country nutrition scientist recommends it is one of ten kind of best foods, also was our country medical department in 1996 announces it for the medicine, the food dual purpose plant. The long handsome Chinese flowering quince is a improved seed of the wrinkle Chinese flowering quince (Chaenomeles lagenaria (Loisel) Koidz.) which is the rose family (Rosaceae) the Chinese flowering quince to be (Chaenomeles Lind), the fallen leaf bush, is the collection watches, for medicinal purposes and edible to a body improved seed.In this paper, the effect of factors on the rooting of long handsome Chinese flowering quince (Chaenomeles lagenaria) with its stems as explants was studied. The growth vigorous and healthy roots have been induced successfully. Also it has established the growth vigorous and healthy long handsome Chinese flowering quince test tube seedling raise system. At the same time, the somatic embryo was induced through direct and indirect methods with the leaf blade as explants. Moreover, it has carried on the preliminary exploration on the somatic cell embryo growth. The studies had been settled the foundation for the fast promoted use in the production and the next development for somatic cell crossbreeding. Has mainly obtained below the conclusion through the experiment:1. Experiments for method of explants'disinfection when the explants disinfects, the leaf blade from petiole's among trims off, stays behind section of petioles on the branch, vaccinates to sprouting on the culture medium to induce sprouts may effective enhance the bud sprouting rate.2. Research on the rooting of long handsome Chinese flowering quince test tube seedling2.1 The appropriate medium for rooting is 1/2MS+NAA8.0 mg/L+AC0.05% alone to increase NAA and 1/2MS+IBA 4.0 mg/L is the suitable medium with IBA. The root rate is high and the quality of the roots is well in the two medium.2.2 Number of leaves,IBA and NAA have remarkable or extremely remarkable influence on the rooting rate. The interaction between the number of leaves and NAA or IBA is not remarkable, but it is remarkable between NAA and IBA. The studies showed that the appropriate number of leaves was 6-8 and the optimal medium was 1/2MS+IBA 2.0mg/L+NAA 4.0mg/L+sucrose 3.0%+agar 0.6%.3. Preliminary study on the somatic embryo of long handsome Chinese flowering quince3.1 The appropriate medium for callus induction is MS + 6-BA 1.0 mg/L + 2, 4-D 0.2mg/L with leaves as explants, darkness is useful.