Genetic Analysis And Mapping Of Yellow-seeded Gene Of Brassica Napus NO.2127-17

The materials used in this study was yellow-seeded rapeseed (Brassica napus) No.2127-17 and black-seeded rapeseed (Brassica napus) was zhongyou821. The inheritance of the yellow-seeded trait for No.2127-17 was studied. AFLP technique was adapted to detect molecular markers linked to the yellow-seeded gene, and the three AFLP markers were converted into SCAR marker or CAPS marker. The main results are as follows:1. The inheritance mode of seed colour in No.2127-17 was investigated in the Fo, RF₀, F₁, RF₁ and F₂ of the cross (No.2127-17×zhongyou821). It was indicated that seed colour was under the control of maternal genotype and yellow seed was partially dominant over black, segregation analysis revealed a single gene locus for the partial dominance of the yellow seed colour.2. We used bulked segregant analysis (BSA) and AFLP techniques to identify markers linked to the yellow-seeded gene. Among 708 AFLP primer pairs, 18 AFLP primer pairs showed polymorphisms between the bulks and the parents. Furthermore, 18 AFLP markers were identified in the vicinity of the yellow-seeded gene locus using a 93 individuals DH progeny of the cross (No.2127-17×zhongyou821). The chromosomal region covered a map length of 37.3 cM with an average of 2.2 cM between two adjacent markers. The marker K-C was located in the linkage group 9 (LG-9) of molecular genetic map of Brassica napus.3. We recovered, cloned and sequenced 3 AFLP markers region which were tightly linked to the yellow-seeded gene. Then we used PCR-Walking to isolate genomic sequences flanking the AFLP markers. Based on the sequences, we designed specific PCR primers (20bp-30bp) and then successfully developed H into SCAR marker (H-S), K digested with Kpn I into CAPS makers (K-C) and B digested with Pvu II into dCAPS makers (B-dC).